Effect of anti-biofilm glass–ionomer cement on Streptococcus mutans biofilms

查看全文 作　　者：Su-Ping [1,2]Wang;Yang [1,2]Ge;Xue-Dong [1,2]Zhou;Hockin HK [3]Xu;Michael D [3]Weir;Ke-Ke [1]Zhang;Hao-Hao [1,2]Wang;Matthias [4]Hannig;Stefan [4]Rupf;Qian [4]Li;Lei [1,2]Cheng

机构地区：[1]State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University;[2]Department of Operative Dentistry and Endodontics,West China Hospital of Stomatology, Sichuan University;[3]Biomaterials and Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School;[4]Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, Saarland University

出　　处：《International Journal of Oral Science》索引2016年第8卷第2期,共8页

基　　金：supported by a National Natural Science Foundation of China grant 81372889 (Lei Cheng), 81430011 (Xue-dong Zhou);the Program for New Century Excellent Talents in University (Lei Cheng);a Youth Grant of the Science and Technology Department of Sichuan Province, China 2014JQ0033 (Lei Cheng);the International Science and Technology Cooperation Program of China 2014DFE30180 (Xue-dong Zhou);NIH R01 DE17974 (Hockin HK Xu);a Seed Grant (Hockin HK Xu) from the University of Maryland School of Dentistry 摘　　要：Dental restorative materials with antimicrobial properties can inhibit bacterial colonization, which may result in a reduction of caries at tooth-filling interaction zones. This study aimed to develop antibacterial glass–ionomer cements(GIC) containing a quaternary ammonium monomer(dimethylaminododecyl methacrylate, DMADDM), and to investigate their effect on material performance and antibacterial properties. Different mass fractions(0, 1.1% and 2.2%) of DMADDM were incorporated into the GIC. The flexure strength, surface charge density, surface roughness and fluoride release were tested. A Streptococcus mutans biofilm model was used. Exopolysaccharides(EPS) staining was used to analyze the inhibitory effect of DMADDM on the biofilm matrix. In addition, biofilm metabolic activity, lactic acid metabolism and the expression of glucosyltransferase genes gtf B, gtf C and gtf D were measured. GIC containing 1.1% and 2.2% DMADDM had flexural strengths matching those of the commercial control(P40.1). DMADDM was able to increase the surface charge density but reduced surface roughness(Po0.05). The incorporation of 1.1% and 2.2% DMADDM elevated the release of fluoride by the GIC in the first 2 days(Po0.05). The novel DMADDM-modified GIC significantly reduced biofilm metabolic activity(Po0.05) and decreased lactic acid production(Po0.05). The quantitative polymerase chain reaction(q PCR) results showed that the expression of gtf B, gtf C and gtf D decreased when mass fractions of DMADDM increased(Po0.05). EPS staining showed that both the bacteria and EPS in biofilm decreased in the DMADDM groups. The incorporation of DMADDM could modify the properties of GIC to influence the development of S. mutans biofilms. In this study, we investigated the interface properties of antibacterial materials for the first time. GIC containing DMADDM can improve material performance and antibacterial properties and may contribute to the better management of secondary caries. 关键词：生物膜模型变形链球菌抗菌玻璃离子水表面电荷密度葡糖基转移酶表面粗糙度抗菌性能

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Effect of anti-biofilm glass–ionomer cement on Streptococcus mutans biofilms

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