Integrated transcriptome analysis of human iPS cells derived from a fragile X syndrome patient during neuronal differentiation

查看全文 作　　者：Ping [1]Lu;Xiaolong [2]Chen;Yun [1]Feng;Qiao [1]Zeng;Cizhong [2]Jiang;Xianmin [2]Zhu;Guoping [2,3]Fan;Zhigang [1,4,5]Xue

机构地区：[1]Tongji Stem Cell Research Center,Tongji University School of Medicine,Shanghai 200092,China;[2]Tongji University,School of Life Sciences and Technology,Shanghai 200092,China;[3]Department of Human Genetics,David Geffen School of Medicine,University of California Los Angeles,Los Angeles CA 90095,USA;[4]Translational Center for Stem Cell Research,Tongji Hospital,Department of Regenera-tive Medicine,Tongji University School of Medicine,Shanghai 200065,China;[5]Tongji University Suzhou Institute,Suzhou 215101,China

出　　处：《Science China(Life Sciences)》索引2016年第59卷第11期,共13页

基　　金：supported by National Program on Key Basic Research Project(2015CB964601,2015CB964702);Joint Research Fund for Overseas Chinese,Hong Kong and Macao Young Scholars(31428016);National Natural Science Foundation of China(Key Program 81430026);Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry(Xianmin Zhu);Shanghai Municipal Commission of Health and Family Planning(XBR2013094);Jiangsu Science and Technology Planning Project(BM2014052) 摘　　要：Fragile X syndrome(FXS) patients carry the expansion of over 200 CGG repeats at the promoter of fragile X mental retardation 1(FMR1), leading to decreased or absent expression of its encoded fragile X mental retardation protein(FMRP). However, the global transcriptional alteration by FMRP deficiency has not been well characterized at single nucleotide resolution, i.e., RNA-seq. Here,we performed in-vitro neuronal differentiation of human induced pluripotent stem(iPS) cells that were derived from fibroblasts of a FXS patient(FXS-iPSC). We then performed RNA-seq and examined the transcriptional misregulation at each intermediate stage during in-vitro differentiation of FXS-iPSC into neurons. After thoroughly analyzing the transcriptomic data and integrating them with those from other platforms, we found up-regulation of many genes encoding TFs for neuronal differentiation(WNT1, BMP4,POU3F4, TFAP2 C, and PAX3), down-regulation of potassium channels(KCNA1, KCNC3, KCNG2, KCNIP4, KCNJ3, KCNK9,and KCNT1) and altered temporal regulation of SHANK1 and NNAT in FXS-iPSC derived neurons, indicating impaired neuronal differentiation and function in FXS patients. In conclusion, we demonstrated that the FMRP deficiency in FXS patients has significant impact on the gene expression patterns during development, which will help to discover potential targeting candidates for the cure of FXS symptoms. 关键词：fragile X syndrome induced pluripotent stem cells neuronal differentiation TRANSCRIPTOME

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Integrated transcriptome analysis of human iPS cells derived from a fragile X syndrome patient during neuronal differentiation

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