Short-term serum deprivation causes no significant mitochondrial DNA mutation in vascular smooth muscle cells revealed by a new next generation sequencing technology

查看全文 作　　者：Xiangqi [1]Li;Lianyong [2,3]Liu;Qian [1]Xi;Xuemei [1]Zhao;iingshuang [1]Fang;Junhua [1]Ma;[1]ZhaohuiZhu;Xing [1]Wang;Chao [1]Shi;Jingnan [1]Wang;Hongling [1]Zhu;Jichen [1]Zhang;Chaobao [4]Zhang;Shuanggang [5]Hu;Minjie [4]Ni;Mingjun [1]Gu 机构地区：[1]Department of Endocrine, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai 200135, China;[2]Medical College of Soochow University, Suzhou, Jiangsu 215123, China;[3]Department of Endocriology, Punan Hospital of Pudong New District, Shanghai 200125, China;[4]Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Shanghai 200031, China;[5]Shanghai Key Laboratory for Assisted Reproduction and Reproductive Benetics, Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200135, China 出　　处：《Acta Biochimica et Biophysica Sinica》索引2016年第48卷第9期,共3页 基　　金：This work was supported by the grants from Shanghai Health and Family Planning Commission (Nos. 201540393, 201440050, and 20134Y055), Shanghai Science and Technology Committee (Nos. 15ZR1437100 and 13ZR1460900), the National Natural Science Foundation of China (Nos. 81500655 and 31301225), the Scientific Research Project of Shanghai Pudong New Area Gongli Hospital (2014GLNQ09). 摘　　要：线粒体提供许多精力因为在线粒体的房间和混乱能几乎影响任何织物[1 ] 。mitochondrial DNA (mtDNA ) 盖住 16,569 bp,由在人的 37 基因和 1 个 D 环区域组成。线粒体在拥有他们被采用 multiprotein 机械组织成 mitochondrial nucleoids 的自己的染色体是唯一的,并且能执行基因复制,抄写,和修理的功能[2 ] 。尽管它让几乎所有已知的原子 DNA 修理小径,保护的 histones 的缺乏可以导致特别危险性到压力和 DNA 损坏事件[3 ] 。确实, mtDNA 让一个很高的变化评价[4 ] 。自从它的发现, mtDNA 变化被知道了普通并且包括糖尿病,心血管的疾病,癌症,神经与肌的混乱,和老化作为人的疾病的一个重要原因出现了,在 4300 人口影响 1 [5,6 ] 。因此,在压力和疾病识别 mtDNA 变化现在是一个热研究区域。