SUMO E3 Ligases GmSIZ1a and GmSIZ1b regulate vegetative growth in soybean

查看全文 作　　者：Bin [1]Cai;Xiangxiong [1]Kong;Chao [2]Zhong;Suli [2]Sun;Xiao Feng [3]Zhou;Yin Hua [1]Jin;Youning [4]Wang;Xia [4]Li;Zhendong [2]Zhu;Jing Bo [1]Jin

机构地区：[1]Key Laboratory of Plant Molecular Physiology,Institute of Botany,The Chinese Academy of Sciences;[2]National Key Facility for Crop Gene Resources and Genetic Improvement,Institute of Crop Science,Chinese Academy of Agricultural Sciences;[3]Department of Ornamental Horticulture,China Agricultural University;[4]State Key Laboratory of Agricultural Microbiology,College of Plant Science and Technology,Huazhong Agricultural University

出　　处：《Journal of Integrative Plant Biology》索引2017年第59卷第1期,共13页

基　　金：supported by grants from the National Natural Science Foundation of China (31471363 for J.B.J.);the Ministry of Science and Technology of the People’s Republic of China (2012CB114302 for J.B.J.);the National Transgenic Major Program (2009ZX08009-087B for J.B.J.and 2009ZX08009-132B for X.L.);the Chinese Academy of Sciences (XDA08010105 for J.B.J.) 摘　　要：SIZ1 is a small ubiquitin-related modifier(SUMO) E3 ligase that mediates post-translational SUMO modification of target proteins and thereby regulates developmental processes and hormonal and environmental stress responses in Arabidopsis. However,the role of SUMO E3 ligases in crop plants is largely unknown. Here, we identified and characterized two Glycine max(soybean) SUMO E3 ligases, GmSIZ1a and GmSIZ1b. Expression of GmSIZ1a and GmSIZ1b was induced in response to salicylic acid(SA), heat, and dehydration treatment, but not in response to cold, abscisic acid(ABA), and Na Cl treatment. Although GmSIZ1a was expressed at higher levels than GmSIZ1b, both genes encoded proteins with SUMO E3 ligase activity in vivo.Heterologous expression of GmSIZ1a or GmSIZ1b rescued the mutant phenotype of Arabidopsis siz1-2, including dwarfism, constitutively activated expression of pathogen-related genes, and ABA-sensitive seed germination.Simultaneous downregulation of GmSIZ1a and GmSIZ1b(GmSIZ1a/b) using RNA interference(RNAi)-mediated gene silencing decreased heat shock-induced SUMO conjugation in soybean. Moreover, GmSIZ1 RNAi plants exhibited reduced plant height and leaf size. However,unlike Arabidopsis siz1-2 mutant plants, flowering time and SA levels were not significantly altered in GmSIZ1 RNAi plants. Taken together, our results indicate that GmSIZ1a and GmSIZ1b mediate SUMO modification and positively regulate vegetative growth in soybean. 关键词：SUMO 营养生长连接酶大豆矮化突变体热休克诱导致病相关基因 RNAi

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SUMO E3 Ligases GmSIZ1a and GmSIZ1b regulate vegetative growth in soybean

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