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The anti-inflammatory effects of asiatic acid in lipopolysaccharide-stimulated human corneal epithelial cells

查看全文 作　　者：Hao [1]Chen;Xiao-Min [1]Hua;Bai-Chen [1]Ze;Bin [1]Wang;Li [1]Wei 高影响力作者 机构地区：[1]Special Medicine Department of Medical College,Qingdao University,Qingdao 266071,Shandong Province,China高影响力机构 出　　处：《International Journal of Ophthalmology(English edition)》索引2017年第10卷第2期,共7页高影响力期刊 基　　金：Supported by Medical Program of Shandong Province(No.2014WS0441);Science and Technology Program of Shandong Province(No.2013YD21009);Innovative Team and Young Teachers Training Project of Qingdao Medical College(No.600201304) 摘　　要：AIM: To investigate the anti-inflammatory effects of asiatic acid(AA) on lipopolysaccharide(LPS)-induced inflammatory response in human corneal epithelial cells(HCECs).METHODS: Cell viability was measured using a cell counting kit-8(CCK-8) assay.Quantitative real-time polymerase chain reaction(qR T-PCR) was used to determine the mR NA expression of interleukin-8(IL-8),interleukin-6(IL-6),interleukin-1β(IL-1β),tumor necrosis factor-alpha(TNF-α),and transforming growth factor-β(TGF-β) in HCECs.Intracellular reactive oxygen species(ROS) was measured using the ROS assay kit.Glutathione(GSH) concentration was measured using the total GSH assay kit.Akt1 and Akt phosphorylation(p-Akt1) levels were measured by Western blotting and immunofluorescence.RESULTS: AA induced toxicity at high concentrations and significantly stimulated the proliferation of HCECs at concentrations of 20 μmol/L for 1h.LPS at concentrations of 300 ng/mL for 1h significantly stimulated the mR NA expression of IL-8,IL-6,IL-1β,TNF-α,and TGF-β in HCECs,while the stimulation effects were significantly inhibited by AA(20 μmol/L).In addition,AA was found to decrease the content of ROS,increase GSH generation,and also inhibit LPS-induced p-Akt in HCECs.CONCLUSION: AA decreases the generation of inflammatory factors IL-8,IL-6,IL-1β,TNF-α,and TGF-β in LPSstimulated HCECs.AA significantly inhibites the intracellular concentrations of ROS and increases GSH generation.AA also inhibites LPS-induced p-Akt in HCECs.These findings reveal that AA has anti-inflammation effects in LPS-stimulated HCECs. 关 键 词：asiatic 酸 LIPOPOLYSACCHARIDE 煽动性的因素 反应的氧种类 谷胱甘肽 Akt phosphorylation
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