Quantitative determination of AI-2 quorum-sensing signal of bacteria using high performance liquid chromatography–tandem mass spectrometry

查看全文 作　　者：Fang [1,2]Xu;Xiangning [1]Song;Peijie [1]Cai;Guoping [1]Sheng;Hanqing [1]Yu

机构地区：[1]CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science & Technology of China;[2]School of Medical Engineering, Hefei University of Technology

出　　处：《Journal of Environmental Sciences》索引2017年第29卷第2期,共6页

基　　金：supported by the National Natural Science Foundation of China(Nos.21261160489,21207031 and51538011);the Program for Changjiang Scholars and Innovative Research Team in University,China 摘　　要：Autoinducer 2(AI-2), an important bioactive by-product of the Lux S-catalyzed S-ribosylhomocysteine cleavage reaction in the activated-methyl-cycle, has been suggested to serve as a universal intra- and inter-species signaling molecule. The development of reliable and sensitive methods for quantitative determination of AI-2 is highly desired.However, the chemical properties of AI-2 cause difficulty in its quantitative analysis.Herein, we report a high performance liquid chromatography-tandem mass spectrometric method that enables reproducible and sensitive measurement of AI-2 concentrations in complex matrixes. 4,5-Dimethylbenzene-1,2-diamine(DMBDM), an easy-to-obtain commercial reagent, was used for the derivatization treatment. The assay was linear in the concentration range of 1.0–1000 ng/m L(R^2= 0.999) and had a lower limit of quantification of0.58 ng/m L. The method exhibited several advantages, e.g., high selectivity, wide linear response range, and good sensitivity. Furthermore, the effectiveness of the method was further validated through measuring AI-2 concentrations in the cell-free culture supernatant from Escherichia coli wild type. 关键词：液相色谱-串联质谱法定量测定信号分子高效液相色谱群体感应细菌浓度测量线性关系

相关文献

参考文献（17）
引证文献（10）
耦合文献（0）

参考文献(17)

引证文献(10)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Quantitative determination of AI-2 quorum-sensing signal of bacteria using high performance liquid chromatography–tandem mass spectrometry

参考文献(17)

引证文献(10)

用户提示