Penicillin-resistant characterization of Staphylococcus aureus isolated from bovine mastitis in Gansu,China

查看全文 作　　者：YANG [1]Feng;LIU Long-[1]hai;WANG [1]Ling;WANG Xu-[1]rong;LI Xin-[1]pu;LUO Jin-[1]yin;ZHANG [1]Zhe;ZHANGShi-[1]dong;YAN Zuo-[1]ting;LI Hong-[1]sheng

机构地区：[1]Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Lanzhou 730050,P.R.China

出　　处：《Journal of Integrative Agriculture》索引2017年第16卷第8期,共5页

基　　金：supported by the Central Public-Interest Scientific Institution Basal Research Fund,China(1610322015007);the Key Technologies R&D Program of China during the 12th Five-year Plan period(2012BAD12B03);the Natural Science Foundation of Gansu Province,China(145RJYA311) 摘　　要：Bovine mastitis caused by Staphylococcus aureus is difficult to treat because of increasing resistance against antibiotics, especially penicillin. β-Lactamase and biofilm are responsible for penicillin resistance of S. aureus. The aim of this study was to investigate the β-lactamase activity and biofilm formation capacity of 37 penicillin-resistant S. aureus strains(35 were blaZ positive and 2 were blaZ negative) from bovine mastitis in Gansu Province, China, as well as to measure the intercellular adhesion genes icaA and icaD of these strains. β-Lactamase Test Kit was used to determine the β-lactamase activity, biofilm formation was tested by semi-quantitative adherence assay method. Moreover, the presence of icaA and icaD were measured by PCR. A total of 32 penicillin-resistant S. aureus strains, including the two blaZ-negative strains, were identified as β-lactamase producers. All tested S. aureus isolates produced biofilm in the microtiter plate assay. Meanwhile, all these strains were PCR-positive for the ica locus, icaA and icaD. The study indicated high prevalence of β-lactamase activity, biofilm-forming capacity, and the ica genes among the penicillin-resistant S. aureus isolates, and implied that S. aureus resistant to penicillin was attributed to multiple mechanisms. 关键词：金黄色葡萄球菌抗生素耐药性奶牛乳腺炎青霉素甘肃省中国 Β-内酰胺酶检测试剂盒

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Penicillin-resistant characterization of Staphylococcus aureus isolated from bovine mastitis in Gansu,China

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