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Expression of Invertase Genes in Virus-free Sugarcane

查看全文 作  者:Jungang [1]WANG;Tingting [1]ZHAO;Benpeng [1]YANG;Wenwei [1]CAI;Cuilian [1]FENG;Jun [1]ZENG;Guoru [1]XIONG;Shuzhen [1]ZHANG 高影响力作者 机构地区:[1]Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of agriculture/Sugarcane Research Center of Chinese Academy of Tropical Agricultural Sciences/Institute of Tropical Bioscience and Biotechnology of Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China高影响力机构 出  处:《Agricultural Biotechnology》索引2017年第6卷第4期,共6页高影响力期刊 基  金:Supported by'863'Program(2013AA102604-1);Natural Science Foundation of Hainan Province(20163124);Basal Research Fund for Central Public-interest Scientific Institute(ITBB140503);Earmarked Fund for China Agriculture Research System(CARS-20-2-5) 摘  要:Virus-free sugarcane seedlings have improved biomass and sucrose content compared with ordinary seedlings,and sucrose invertases are key enzymes regulating sugarcane growth and sucrose accumulation.In this study,the differences in the expression levels of 3 invertase genes,CWI,SAI and NI,between virusfree and ordinary sugarcane seedlings were analyzed.Compared with ordinary sugarcane plants,the expression of CWI was mainly up-regulated in immature leaves and stems at elongation stage and leaves and immature internodes at maturation stage,and especially,greatly up-regulated in immature internodes at maturation stage of virus-free plants.The expression of SAI and NI were mainly up-regulated in leaves and immature internodes of virus-free plants at maturation stage,which might be beneficial to sugar accumulation and rapid utilization of monosaccharide in the stalks of virus-free plants.It is further indicated that virus-free treatment could significantly improve the expression of sucrose invertases at late growth period,and might facilitate the increase of plant biomass. 关 键 词:基因表达水平 蔗糖转化酶 无病毒 甘蔗 植物生物量 植物叶片 脱毒处理 糖分积累
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