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Combination of Quercetin, Cinnamaldehyde and Hirudin Protects Rat Dorsal Root Ganglion Neurons against High Glucose-Induced Injury through Nrf-2/HO-1 Activation and NF-κB Inhibition

查看全文 作　　者：SHI [1]Yue;LIANG Xiao-[1]chun;ZHANG [2]Hong;SUN [1]Qing;WU Qun-[1]li;QU [1]Ling 高影响力作者 机构地区：[1]Department of Traditional Chinese Medicine, Translational Medicine Center, Peking Union Medical College Hospital,Chinese Academy of Medical Sciences;[2]Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College高影响力机构 出　　处：《Chinese Journal of Integrative Medicine》索引2017年第23卷第9期,共9页高影响力期刊 基　　金：the office of Traditional Chinese Medicine of Peking Union Medical College Hospital;the Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences, Pecking, China, for supporting this research. 摘　　要：Objective: To examine the effects of the combination of quercetin(Q), cinnamaldehyde(C) and hirudin(H), a Chinese medicine formula on high glucose(HG)-induced apoptosis of cultured dorsal root ganglion(DRG) neurons. Methods: DRG neurons exposed to HG(45 mmol/L) for 24 h were employed as an in vitro model of diabetic neuropathy. Cell viability, reactive oxygen species(ROS) level and apoptosis were determined. The expression of nuclear factor of Kappa B(NF-κB), inhibitory kappa Bα(IκBα), phosphorylated IκBα and Nf-E2 related factor 2(Nrf2) were examined using reverse transcription-polymerase chain reaction(RT-PCR) and Western blot assay. The expression of hemeoxygenase-1(HO-1), interleukin-6(IL-6), tumor necrosis factor(TNF-α) and caspase-3 were also examined by RT-PCR and Western blot assay. Results: HG treatment markedly increased DRG neuron apoptosis via increasing intracellular ROS level and activating the NF-κB signaling pathway(P<0.05). Co-treatment with Q, C, H and their combination decreased HG-induced caspase-3 activation and apoptosis(P<0.05 or P<0.01). The expressions of NF-κB, IL-6 and TNF-α were downregulated, and Nrf2/HO-1 expression was up-regulated(P<0.05 or P<0.01). QCH has better effect in scavenging ROS, activating Nrf-2/HO-1, and down-regulating the NF-κB pathway than other treatment group. Conclusions: DRG neurons' apoptosis was increased in diabetic conditions, which was reduced by QCH formula treatment. The possible reason could be activating Nrf-2/HO-1 pathway, scavenging ROS, and inhibition of NF-κB activation. The effect of QCH combination was better than each monomer or the combination of the two monomers. 关 键 词：背根神经节神经元 诱导培养 水蛭素 槲皮素 肉桂醛 活化 caspase-3 逆转录-聚合酶链反应
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