Immune fluorescence test strips based on quantum dots for rapid and quantitative detection of carcino-embryonic antigen

查看全文 作　　者：Yudong [1]Wu;Weipan [1]Peng;Qian [1]Zhao;Jiafang [1]Piao;Bo [1]Zhang;Xiaoli [1]Wu;Hanjie [1]Wang;Zhihong [2]Shi;Xiaoqun [1]Gong;Jin [1]Chang

机构地区：[1]School of Life Sciences,School of Materials Science and Engineering,Tianjin University and Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology(Tianjin);[2]Department of Neurology,and Tianjin Neurosurgery Institute,Tianjin Huanhu Hospital

出　　处：《Chinese Chemical Letters》索引2017年第28卷第9期,共4页

基　　金：financially supported by the National Natural Science Foundation of China(Nos.51373117,51303126 and 31600800);Tianjin Natural Science and Technology Foundation(No.16ZXMJSY00010) 摘　　要：At present,many researchers focused on the point-of-care testing(POCT),a method of disease markers detection without large-scale instruments and specialized persons.However,most POCT diagnostic methods were suffered from poor detection sensitivity or inefficiency in quantitative detection.Herein,we developed a newly QD-immune fluorescence test strips(QD-IFTS) based on quantum dots(QDs) as the fluorescence nanocarrier to prepare the immune fluorescence probes in the classical immunochromatography detection system for sensing carcino-embryonic antigen(CEA),a kind of glycoprotein produced by intestinal tissue and a broad spectrum of tumor marker for cancer diagnosis.And we designed a homemade strips fluorescence reader for detection of fluorescence intensity of QDs on the QD-IFTS.Under the optimized reaction conditions,chromatographic time of the newly QD-IFTS was only25 min,sample volume of the newly QD-IFTS was only 40 m L and the LOD of the newly QD-IFTS was 0.72 ng/m L.In addition,the efficiency and robustness of the newly QD-IFTS were confirmed by successfully application in 300 clinical serum samples,and the results revealed great potential in clinical POCT of other biomarkers. 关键词：免疫荧光检测抗原检测癌胚抗原量子点测试点肿瘤标志物纸基诊断方法

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Immune fluorescence test strips based on quantum dots for rapid and quantitative detection of carcino-embryonic antigen

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