维普中文期刊产品整合服务

The oral commensal Streptococcus mitis activates the aryl hydrocarbon receptor in human oral epithelial cells

查看全文 作　　者：stian a [1]engen;gro h [1]rørvik;olav [1]schreurs;inger js [1,2]blix;karl [1]schenck 高影响力作者 机构地区：[1]department of oral biology,faculty of dentistry,university of oslo,Oslo,Norway;[2]institute of clinical dentistry,faculty of dentistry,university of oslo,Oslo,Norway高影响力机构 出　　处：《International Journal of Oral Science》索引2017年第9卷第3期,共6页高影响力期刊 基　　金：supported by the Norwegian Research Council grant no.241011;the Norwegian Dental Depot Fund for Dental Research 摘　　要：Streptococcus mitis(S. mitis) is a pioneer commensal bacterial species colonizing many of the surfaces of the oral cavity in healthy individuals. Yet, not much information is available regarding its interaction with the host. We used examination of its transcriptional regulation in oral keratinocytes to elucidate some of its potential roles in the oral cavity. Transcription factor analysis of oral keratinocytes predicted S. mitis-mediated activation of aryl hydrocarbon receptor(Ah R). Activation and functionality of Ah R was confirmed through nuclear translocation determined by immunofluorescence microscopy and real-time polymerase chain reaction with reverse transcription analysis of CYP1A1, the hallmark gene for Ah R activation. Addition of Streptococcus mutans or Streptococcus gordonii did not induce CYP1A1 transcription in the keratinocyte cultures. Introduction of an Ah R-specific inhibitor revealed that S. mitis-mediated transcription of CXCL2 and CXCL8 was regulated by Ah R. Elevated levels of prostaglandin E2(enzyme-linked immunosorbent assay) in supernatants from S. mitis-treated oral epithelial cells were also attenuated by inhibition of Ah R activity. The observed Ah R-regulated activities point to a contribution of S. mitis in the regulation of inflammatory responses and thereby to wound healing in the oral cavity. The concept that the oral commensal microbiota can induce Ah R activation is important, also in view of the role that Ah R has in modulation of T-cell differentiation and as an anti-inflammatory factor in macrophages. 关 键 词：口腔修复 变形链球菌 上皮细胞 共生细菌 芳香烃 受体 激活 角质形成细胞
相关文献

参考文献(32)

引证文献(2)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费