Molecular cloning and structural analysis of human norepinephrine transporter gene(NETHG)

查看全文 作　　者：GUO LIHE;LIHUA ZHU;FANG HUANG;ANTHONY CW TAM;ZENGCHAN YE;JIAN FEI;XIAOYONG ZHANG;DOMINIC MAN-KIT LAM (Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.)(Honq Kong Institute of Biotechnology, Shatin, NT,Hong Ko 出　　处：《Cell Research》索引1995年第5卷第1期,共8页 摘　　要：A cDNA molecule encoding a major part of the hu-man Norepinephrine transporter(hNET) was synthesized by means of Polymerase Chain Reaction(PCR) technique and used as a probe for selecting the human genomic NET gene. A positive clone harbouring the whole gene was ob-tained from a human lymphocyte genomic library through utilizing the 'genomic walking' technique. The clone, des-ignated as phNET, harbours a DNA fragment of about 59 kb in length inserted into BamH Ⅰ site in cosmid pWE15.The genomic clone contains 14 exons encoding all amino acid residues in the protein. A single exon encodes a dis-tinct transmembrane domaill, except for transmembrane domain 10 and 11, which are encoded by part of two ex-ons respectively, and exon 12, which encodes part of do-main 11 and all of domain 12. These results imply that there is a close relationship between exon splicing of a gene and structural domains of the protein, as is the case for the human γ-aminobutyric acid transporter(hGAT) and a number of other membrane proteins. 关 键 词：NETHG 人去甲肾上腺素转运蛋白基因 分子克隆结构分析 神经递质