Apoptosis of human leukemic HL-60 cells induced to differentiate by treatment with RA or DMSO

查看全文 作　　者：[1]SUNHONG;[2]YONGCHAOWANG 机构地区：[1]KeyLaboratoryofCellProliferationandRegulationBiology,BeijingNormalUniversity,Beijing100875,China;[2]KeyL 出　　处：《Cell Research》索引1995年第5卷第2期,共6页 摘　　要：In present study we studied the effect of all-trans retinoic acid(ATRA) and dimethylsulfoxide (DMSO) on the induction of apoptosis in HL-60 cell line. Based on morphological changes by Hochest 33342 staining and identification of internucleosomal DNA cleavage by gel electrophoresis, we observed aberrant nuclear chromatin eondensation and ladder-like pattern of DNA degradation. Using Flow Cytometric method, we found sub-G1 peak in RA-treated HL-60 cells starting 5 to 6 d after the initiation of the treatment. However, such an obvious apoptotic peak was not identified in DMSO-differentiated cells. Combining the research accomplished before, our study approves further that apoptosis could be a common mode of death of terminally differentiated HL-60 cells. 关 键 词：人类白血病 HL-60细胞 全反式核苷酸 处理 诱发分化 细胞凋亡