Cultivation of Hippeastrum vittatum Embryogenic Calli and Their Sensitivity to Antibiotics

查看全文 作　　者：Bo [1]YU;Min [2]LIU;Yu [3]ZHU;Aidong [4]ZHONG;Lili [1]HUANG;Dongxiang [4]ZHU;Chunhui [2]LI;Genfa [1]ZHU;Yingbo [1]SUN

机构地区：[1]Key Laboratory of Urban Agriculture in South China, Ministry of Agriculture/Guangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;[2]Lingnan Landscape Co. , Ltd. Dongguan 510600, China;[3]Chinese Bioresource Application Association, Taiwan 26047;[4]Guangzhou Hengtai Agricultural Economy Development Co. ,Ltd, Guangzhou 510800, China

出　　处：《Agricultural Biotechnology》索引2018年第7卷第1期,共4页

基　　金：Supported by Science and Technology Program of Guangzhou City(2016201604030076);Science and Technology Program of Guangdong Province(2014A020208062);Cooperation Project of Industry,Education and Academy of Dongguan City(2015509105105);the Fund for Demonstration and Extension of Introduced Intellectual Achievements in Guangdong 'Forcing Cultivation and Flowering Regulation Technology Introduction,Demonstration and Promotion in Hippeastrum vittatum' and 'The Characteristics of Taiwan Varieties and Cultivation Technology Introduction and Promotion in Hippeastrum vittatum';Open Project of Key Laboratory of Urban Agriculture in South China,Ministry of Agriculture,China 摘　　要：In this study,an embryogenic callus induction and proliferation system for Hippeastrum vittatum was established,with the tender bulbs as explants. And then the sensitivity of the explants and calli to kanamycin and hygromycin was evaluated. The results suggested that the embryogenic calli were induced from tender bulbs and cultured in Murashige and Skoog( MS) medium supplemented with 0. 5 mg/L N-phenyl-N'-1,2,3-thiadiazol-5-ylurea( TDZ),1. 0 mg/L 2,4-dichlorophenoxyacetic acid( 2,4-D),and 30 g/L sucrose( pH5. 8) in the dark at 25 ± 1 ℃. Further study of the influence of kanamycin and hygromycin on callus induction and multiplication showed that,the lethal doses of kanamycin and hygromycin to bulb explants were 100 and 30 mg/L,respectively. All explants of H. vittatum died on the medium supplemented with 100 mg/L kanamycin or 30 mg/L hygromycin at the induction stage,and callus proliferation was completely inhibited by 100 mg/L kanamycin or 25 mg/L hygromycin,and all the calli died at last. These results will provide important reference for further studies of transgenic H. vittatum. 关键词：敏感抗菌素组织移植耕作培养基生物灯泡媒介

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Cultivation of Hippeastrum vittatum Embryogenic Calli and Their Sensitivity to Antibiotics

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