Cepharanthine enhance radiosensitization of nasopharyngeal carcinoma cells through inhibition of DNA damage repair

查看全文 作　　者：Mengya [1]Zhang;Tianjun [2]Ma;Qingfeng [1]Song;Huixia [1]Lin;Wei [1]Xiao;Meiqi [1]Zhao;XinQing [1]Liang;Ying [1]Cui

机构地区：[1]Department of Medical Research, Affiliated Tumor Hospitalof Guangxi Medical University,Nanning 530021, P.R. China;[2]Department of Orthopedics, First Affiliated Hospital ofGuangxi Medical University, Nanning 530021, P.R. China

出　　处：《广西医科大学学报》索引2018年第35卷第2期,共5页

基　　金：supported by the National Natural Science Foundation of China (No. 30660203);the National Science and Technology Major Project (No. 2013ZX10002009);the Key laboratory of High- Incidence Tumor Prevention (Guangxi Medical University),Ministry of Education(No. GK2015-ZZ06) 摘　　要：Objective: Recent reports suggest that Cepharanthine(CEP),a natural alkaloid extracted from the roots of Stephania Cepharanthine Hayata,can inhibit the proliferation of various cancer cells,but few studies focus on its radiosensitization in nasopharyngeal carcinoma(NPC)cells. The aim of this study was to explore the radiosensitization effect and the potential mechanisms of CEP on nasopharyngeal carcinoma cell lines CNE-1 and CNE-2. Methods: The NPC cell lines CNE-1 and CNE-2 were treated with CEP(IC50)for 48 h before irration(IR);clonogenic survival was then assessed. The apoptosis and cell cycle progression were using flow cytometry.The DNA damage repair and cycle-regulating proteins were evaluated by Western blot analysis. Results: CEP could inhibit cell growth in both cell lines. The combination of CEP and radiation promote cell cycle G2/M phase arrest and apoptosis in CNE-1 and CNE-2 cells.DNA damage repair analysis showed that CEP has an inhibitory effect on DNA repair of CNE-1 and CNE-2 cells after radiation.Conclusion: CEP enhances tumor radioresponse through multiple mechanisms that may involve the halted cell cycle progression at G2/M phase and the inhibition of DNA repair after exposure to radiation. 关键词：《广西医科大学学报》期刊编辑工作发行工作

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Cepharanthine enhance radiosensitization of nasopharyngeal carcinoma cells through inhibition of DNA damage repair

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