Desiccation tolerance in bryophytes: the rehydration proteomes of Bryum argenteum provide insights into the resuscitation mechanism

查看全文 作　　者：GAO [1,2]Bei;ZHANG [1]Daoyuan;LI [1]Xiaoshuang;YANG [1]Honglan;LIANG [1]Yuqing;CHEN [2]Moxian;ZHANG [1]Yuanming;ZHANG [2,3]Jianhua;Andrew [4]WOOD

机构地区：[1]Key Laboratory of Biogeography and Bioresource in Arid Land,Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,China;[2]School of Life Sciences and State Key Laboratory of Agrobiotechnology,The Chinese University of Hong Kong,Hong Kong,China;[3]Department of Biology,Faculty of Science,Hong Kong Baptist University,Hong Kong,China;[4]Department of Plant Biology,Southern Illinois University-Carbondale,Carbondale,IL 62901-6509,USA

出　　处：《Journal of Arid Land》索引2018年第10卷第1期,共16页

基　　金：supported by the Scientific Service Project of Chinese Academy of Sciences (TSS-2015-014-FW-4-3);the National Science Foundation of China-Xinjiang Talent Youth Project (U1403302) 摘　　要：Bryum argenteum Hedw. is a desiccation tolerant bryophyte and belongs to one of the most important components of the biological soil crusts(BSCs) found in the deserts of Central Asia. Limited information is available on rehydration-responsive proteins in desiccation tolerant plants. As a complement to our previous research analyzing the rehydration transcriptome, we present a parallel quantitative proteomic effort to study rehydration-responsive proteins. Bryophyte gametophores were desiccated(Dry) and rehydrated for 2 h(R2) and 24 h(R24). Proteins from Dry, R2 and R24 gametophores were labeled by isobaric tags for relative and absolute quantitation(iTRAQ) to determine the relative abundance of rehydration-responsive proteins. A total of 5503 non-redundant protein sequences were identified and 4772(86.7%) protein sequences were annotated using Gene Ontology(GO) terms and Pfam classifications. Upon rehydration 239 proteins were elevated and 461 proteins were reduced as compared to the desiccated protein sample. Differentially up-regulated proteins were classified into a number of categories including reactive oxygen species scavenging enzymes, detoxifying enzymes, Late Embryogenesis Abundant(LEA) proteins, heat shock proteins, proteasome components and proteases, and photosynthesis and translation related proteins. Furthermore, the results of the correlation between transcriptome and proteome revealed the discordant changes in the expression between protein and mRNA. 关键词：干燥植物苔类析水蛋白质序列 PROTEOME 机制复活

相关文献

参考文献（56）
引证文献（2）
耦合文献（71）

参考文献(56)

引证文献(2)

耦合文献(71)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Desiccation tolerance in bryophytes: the rehydration proteomes of Bryum argenteum provide insights into the resuscitation mechanism

参考文献(56)

引证文献(2)

耦合文献(71)

用户提示