Cytotoxic effect of interleukin-8 in retinal ganglion cells and its possible mechanisms

查看全文 作　　者：Jing-Jing [1]Wang;Walana [1]Williams;Bing [1]Wang;Jing [1]Wei;Xia [2]Lu;Jya-Wei [3]Cheng;John R [4]Gordon;Jing-Min [2]Li;Fang [1]Li

机构地区：[1]Department of Immunology, Dalian Medical University,Dalian 116044, Liaoning Province, China;[2]Department of Ophthalmology, Second Affiliated Hospital of Dalian Medical University, Dalian 116023, Liaoning Province,China;[3]Institute of Biotechnology, Department of Life Science, Tsing Hua University, Hsinchu 300, Taiwan, China;[4]the Division of Respirology, Critical Care and Sleep Medicine,Royal University Hospital, University of Saskatchewan,Saskatoon S7N 5A2, Canada

出　　处：《International Journal of Ophthalmology(English edition)》索引2018年第11卷第8期,共7页

摘　　要：AIM: To investigate the effect of interleukin-8(IL-8) on neural retinal ganglion cells(RGCs) and whether it can be alleviated by G31P. METHODS: RGC-5 cells were exposed to IL-8 with or without its specific receptor antagonist G31P for 24h, and the cell viability was assessed by Cell Counting Kit 8(CCK-8). Apoptosis was measured by examining nuclear morphology and quantifying with flow cytometry. Reverse transcription quantitative real-time polymerase chain reaction(RT-qP CR) and Western blot were used to investigate the expression of apoptosis-related genes. RESULTS: CCK-8 assay showed that IL-8 significantly inhibits the viability of RGC-5 cells in a dose-dependent manner. Cell apoptosis assays exhibited higher apoptotic rate in IL-8 treatment group compared to control group. We further found that IL-8 could promote Bax and caspase-3 expressions, but decrease the level of Bcl-2 in the aspect of m RNA and protein. However, pre-treatment with G31P partly attenuated these effects in RGC-5 cells(P<0.05).CONCLUSION: These results indicate that anti-proliferation effects of IL-8 through induction of cell apoptosis regulated by Bcl-2, Bax and caspase-3 expressions, can be ameliorated by G31P. 关键词：GLAUCOMA INFLAMMATION INTERLEUKIN-8 retinal ganglion cell-5 APOPTOSIS G31P

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Cytotoxic effect of interleukin-8 in retinal ganglion cells and its possible mechanisms

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