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Real-time Microwave Exposure Induces Calcium Efflux in Primary Hippocampal Neurons and Primary Cardiomyocytes

查看全文 作　　者：WANG [1]Hui;ZHANG [1]Jing;HU Shao [1,2]Hua;TAN Sheng [1]Zhi;ZHANG [1]Bo;ZHOU Hong [1]Mei;PENG Rui [1]Yun 高影响力作者 机构地区：[1]Department of Experimental Pathology, Institute of Radiation Medicine, Academy of Military Medical Sciences;[2]Department of Clinical Laboratory, Shanghai Children's Hospital, Shanghai Jiao Tong University高影响力机构 出　　处：《Biomedical and Environmental Sciences》索引2018年第31卷第8期,共11页高影响力期刊 基　　金：funded by the National Natural Science Foundation of China General [Grant No.81172620,No.81402629,and No.61401497];Innovation Foundation of Academy of Military Medical Sciences [2017CXJJ17,2015CXJJ06] 摘　　要：Objective To detect the effects of microwave on calcium levels in primary hippocampal neurons and primary cardiomyocytes by the real-time microwave exposure combined with laser scanning confocal microscopy. Methods The primary hippocampal neurons and primary cardiomyocytes were cultured and labeled with probes, including Fluo-4 AM, Mag-Fluo-AM, and Rhod-2, to reflect the levels of whole calcium [Ca^(2+)], endoplasmic reticulum calcium [Ca^(2+)]ER, and mitochondrial calcium [Ca^(2+)]MIT, respectively. Then, the cells were exposed to a pulsed microwave of 2.856 GHz with specific absorption rate(SAR) values of 0, 4, and 40 W/kg for 6 min to observe the changes in calcium levels. Results The results showed that the 4 and 40 W/kg microwave radiation caused a significant decrease in the levels of [Ca^(2+)], [Ca^(2+)]ER, and [Ca^(2+)]MIT in primary hippocampal neurons. In the primary cardiomyocytes, only the 40 W/kg microwave radiation caused the decrease in the levels of [Ca^(2+)], [Ca^(2+)]ER, and [Ca^(2+)]MIT. Primary hippocampal neurons were more sensitive to microwave exposure than primary cardiomyocytes. The mitochondria were more sensitive to microwave exposure than the endoplasmic reticulum. Conclusion The calcium efflux was occurred during microwave exposure in primary hippocampal neurons and primary cardiomyocytes. Additionally, neurons and mitochondria were sensitive cells and organelle respectively. 关 键 词：Real time Microwave CALCIUM PRIMARY HIPPOCAMPAL neurons PRIMARY CARDIOMYOCYTES
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