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Optimization of enzyme assisted extraction of polysaccharides from pomegranate peel by response surface methodology and their anti-oxidant potential

查看全文 作　　者：Yun [1]Li;Cai-ping [1,2]Zhu;Xi-chuan [1]Zhai;Yang [1]Zhang;Zhen [1]Duan;Jing-ru [1]Sun 高影响力作者 机构地区：[1]College of Food Engineering and Nutrition Science,Shaanxi Normal University,Xi'an 710119,China;[2]International Joint Research Center of Shaanxi Province for Food and Health Sciences,Xi'an 710119,China高影响力机构 出　　处：《Chinese Herbal Medicines》索引2018年第10卷第4期,共8页高影响力期刊 基　　金：supported by National Natural Science Foundation of China(No.31301598);the Fundamental Research Fund for the Central Universities of China(No.GK201803086) 摘　　要：Objective: To optimize the enzymatic extraction conditions of polysaccharides from pomegranate peel using response surface methodology(RSM), and invest the anti-oxidant activity of pomegranate peel polysaccharide in vitro for seeking novel biological components used as pharmaceutical products and functional foods.Methods: Effects of enzymolysis time, ratio of water to raw material, and the dosage of cellulase on the extraction yield of pomegranate peel polysaccharide were fully examined by Box-Behnken design of RSM. DPPH radical scavenging activity, hydroxyl radicals scavenging activity, superoxide anion radical scavenging activity, and reducing power assay were measured by microplate reader.Results: The optimal extraction conditions were as follows: enzymolysis temperature 55 °C, pH 5.0,enzymolysis time 88 min, ratio of water to raw material 22:1 mL/g, and dosage of cellulose 0.93%. Under the optimal extraction conditions, the yield of pomegranate peel polysaccharide was(22.31 ± 0.07)%,which was well matched with the predicted value 22.35% of the Box-Behnken design model. Also,pomegranate peel polysaccharide demonstrated appreciable anti-oxidant potential on DPPH(1,1-diphenyl-2-picrylhydrazyl) radical scavenging, hydroxyl radical scavenging, superoxide anion radical scavenging and reducing power in vitro.Conclusions: An optimized enzymolysis assisted method was proposed for the extraction of pomegranate peel polysaccharide, which can be used as a good anti-oxidant. 关 键 词：抗氧化剂 石榴皮 方法论 多糖 抽取 优化 表面 反应
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