Optimization of microwave-assisted extraction of bioactive alkaloids from lotus plumule using response surface methodology

查看全文 作　　者：Wei [1]Xiong;Xianqiang [1]Chen;Guangping [1]Lv;Dejun [1]Hu;Jing [1]Zhao;Shaoping [1]Li

机构地区：[1]State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macao,Macao SAR

出　　处：《Journal of Pharmaceutical Analysis》索引2016年第6卷第6期,共7页

基　　金：partially supported by grants from the Science and Technology Development Fund of Macao(FDCT059/2011/A3);the University of Macao(MYRG085 to Jing Zhao and MYRG201400041 to LSP,respectively) 摘　　要：In this work,a fast and efficient microwave-assisted extraction(MAE) method was developed to extract main bioactive alkaloids from lotus plumue.To optimize MAE conditions,three main factors were selected using univariate approach experiments,and then central composite design(CCD).The optimal extraction conditions were as follows:methanol concentration of 65%,microwave power of 200 W,and extraction time of 260 s.A high performance liquid chromatography–diode array detector(HPLC–DAD) method was established to quantitatively analyze these phytochemicals in different lotus plumule samples and in different part of lotus.Chromatographic separation was carried out on an Agilent Zorbax Extend-C_(18) column(4.6 mm×150 mm,3.5 μm).Gradient elution was applied with the mobile phase constituted with 0.1% triethylamine in water(A)and acetonitrile(B):40%-70% B at 0-8 min,70%-100% B at 8–9 min,100% B for 2 min,and then equilibrated with 40% B for 2 min. 关键词：LOTUS plumule FUNCTIONAL FOOD MICROWAVE-ASSISTED EXTRACTION ALKALOIDS

相关文献

参考文献（38）
引证文献（4）
耦合文献（0）

参考文献(38)

引证文献(4)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Optimization of microwave-assisted extraction of bioactive alkaloids from lotus plumule using response surface methodology

参考文献(38)

引证文献(4)

用户提示