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A Simple,Efficient and Rapid Method for Good Quality DNA Extraction from Rice Grains

查看全文 作　　者：Abu Ashfaqur [1]SAJIB;Mohammad Ashraful Islam [2]BHUIYA;Roksana [2]HUQUE 高影响力作者 机构地区：[1]Department of Genetic Engineering and Biotechnology,University of Dhaka,Dhaka 1000,Bangladesh;[2]Food Technology Division,Institute of Food and Radiation Biology,Atomic Energy Research Establishment,Savar 1349,Bangladesh高影响力机构 出　　处：《Rice science》索引2017年第24卷第2期,共4页高影响力期刊 基　　金：conducted partly with the Regional Cooperative Agreement project(RAS5062)grant from the International Atomic Energy Agency;the University Grants Commission’s fund(2015–2016)for research projects in the University of Dhaka;International Atomic Energy Agency;the University Grants Commission,Bangladesh for their support。 摘　　要：An efficient and good DNA extraction protocol should be simple, affordable and yield enough DNA with high quality. Rice(Oryza sativa L.) DNA extraction methods often use seedlings or leaves rather than the grains and tend to be time-consuming, involve multiple steps, and use hazardous chemicals and expensive enzymes. Rice grains offer several benefits over seedlings and leaves as a source of DNA for genetic analysis. However, these benefits are underutilized because the bulk of a rice grain is made up of starch. It is particularly important, but difficult to get rid of the starch while extracting DNA from rice grains. This co-precipitated polysaccharide is a known inhibitor of DNA polymerase activity in polymerase chain reaction(PCR). We describe here a very simple and highly affordable Chelex~?-100 based DNA extraction method from rice grains. It does not require any hazardous chemicals or enzymes. This method reproducibly extracts DNA with good purity indices(A_(260)/A_(230) and A_(260)/A_(280) values), but requires only a few steps. 关 键 词：DNA extraction RICE GRAIN Chelex■-100
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