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Identification of In-intro Antagonistic Activity of HAS1 Protein against Sporisorium scitanminea

查看全文 作  者:Huiqing [1]LIU;Yuantao [1]HUANG;Mengyu [1]DAI;Yimin [1]QIU;Gengfeng [1]ZHAO;Guoru [2]XIONG 高影响力作者 机构地区:[1]College of Basic Medicine and Life Science,Hainan Medical University;[2]Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences高影响力机构 出  处:《Agricultural Biotechnology》索引2018年第7卷第6期,共4页高影响力期刊 基  金:Supported by College Students'Innovation and Enterpreneurship Training Program of Hainan Province(2017116);College Students' Innovation and Enterpreneurship Training Program of Hainan Medical University(HYCX2016073);Natural Science Foundation of China(31471555) 摘  要:Sugarcane smut greatly influences yield and quality of sugarcane.HAS1 gene in biocontrol strain HAS antagonistic to Sporisorium scitanminea was selected for prokaryotic expression vector construction and inducible expression,and the antagonistic effect of HAS1 protein was detected in vitro.The results showed that the expression product of antifungal HAS1 gene obtained through prokaryotic expression vector construction and inducible expression had better antagonistic effect against S.scitanminea.This study provides an experimental basis for further utilization of this protein. 关 键 词:BACILLUS SUBTILIS HAS Sporisorium scitanminea PROKARYOTIC expression ANTIFUNGAL protein
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