Diagnosis of Clinical Cases of Infectious Bursal Disease Using a Modified Rapid Taq Man-MGB Real-Time RT-PCR Assay

查看全文 作　　者：Maryame [1]Cheggag;Khalil [2]Zro;Ghizlane [2]Sebbar;Naoufal [3]Rahmatallah;Mohamed [3]Mouahid;Mohammed EL [1]Houadfi;Faouzi [1]Kichou

机构地区：[1]Unit of Histology and Pathological Anatomy,Department of Veterinary Pathology and Public Health,Institute of Agronomy and Veterinary Medicine Hassan II,B.P.6202,Rabat,Morocco;[2]Society of Veterinary Pharmaceutical and Biological Productions(Biopharma),B.P.4569,Rabat,Morocco;[3]Veterinary Clinic Temara,Morocco

出　　处：《Journal of Agricultural Science and Technology(A)》索引2018年第8卷第4期,共9页

摘　　要：Infectious bursal disease(IBD)is an important contagious viral infection of immune system of poultry.This infection possesses a permanent threat to the profitability of poultry industry worldwide.The aim of this work was to modify the Taq Man-MGB real-time reverse transcription-polymerase chain reaction(rRT-PCR)in one step involving two fluorogenic Taq Man labeled probe and using this protocol for detection of infectious bursal disease virus(IBDV)collected from suspected cases distributed in different regions of the country during the period 2013-2016.The intralaboratory validation of modified method was realized for specificity,linearity,repeatability,sensitivity and reproducibility.It allowed reducing the test running time by six folds.This method was applied on 102 pools of bursa of fabricius(BF)samples collected from affected broiler farms suspected to be infected by IBDV.Birds showing macroscopic lesions including muscle petechial hemorrhages,hypertrophy and hemorrhage of BF,were subjected to molecular analysis using modified protocol“Taq Man-MGB rRT-PCR”.The validation satisfied all criteria and the assay developed could be a useful tool for a very rapid diagnosis of IBDV and permit to detect and to discriminate in one-step very virulent(vv)from non-vv(classic and variant)IBDV strains.Out of 84 IBDV positive samples,a prevalence of 39%for vv strains and 61%for classical strains was noted.These results indicate that despite the vaccination against IBDV,the vv form of this pathologie continues to cause serious problems for Moroccan broiler chickens.The obtained results indicate the successfully detection of IBDV and differentiated all vvIBDV strains from non-vvIBDV strains;Avian infectious agent RNA viruses tested are negative,demonstrating great specificity of the assay.The results obtained indicate that this method is suitable as a routine laboratory test for the rapid detection and differentiation of IBDV strains in samples of avian origin. 关键词：IBDV one-step RRT-PCR TAQ Man-MGB macroscopic lesions

相关文献

参考文献（34）
引证文献（0）
耦合文献（3）

参考文献(34)

耦合文献(3)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Diagnosis of Clinical Cases of Infectious Bursal Disease Using a Modified Rapid Taq Man-MGB Real-Time RT-PCR Assay

参考文献(34)

耦合文献(3)

用户提示