Preparation of copolymer-grafted mixed-mode resins for immunoglobulin G adsorption

查看全文 作　　者：Shenggang [1]Chen;Tao [1]Liu;Ruiqi [1]Yang;Dongqiang [1]Lin;Shanjing [1]Yao

机构地区：[1]Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China

出　　处：《Frontiers of Chemical Science and Engineering》索引2019年第13卷第1期,共10页

基　　金：the National Natural Science Foundation of China. 摘　　要：The mixed-mode resins for protein adsorption have been prepared by a novel strategy, copolymer grafting. Specially, the copolymer-grafted resins CG-MA with two functional groups, 5-amino-benzimidazole (ABI) and methacryloxyethyltrimethyl ammonium chloride (METAC), have been prepared through surfaceinitiated activator generated by electron transfer for atom transfer radical polymerization of METAC and glycidyl methacrylate (GMA), followed by a ring-open reaction to introduce ABI. The charge and hydrophobicity of CG-MA resins could be controlled by manipulating the addition of METAC and GMA/ABI. Besides, METAC and ABI provided positive effects together in both protein adsorption and elution: dynamic binding capacity of human Immunoglobulin G (hlgG) onto CG-M-A resin with the highest ligand ratio of METAC to ABI is 46.8 mg-g 1 at pH 9 and the elution recovery of hlgG is 97.0% at pH 5. The separation experiment showed that purity and recovery of monoclonal antibody from cell culture supernatant are 96.0% and 86.5%, respectively, indicating that copolymer-grafted mixed-mode resins could be used for antibody purification. 关键词：atom transfer RADICAL polymerization copolymer-grafting MIXED-MODE resin protein ADSORPTION

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Preparation of copolymer-grafted mixed-mode resins for immunoglobulin G adsorption

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