Development of an innovative one-step nested PCR strategy for virus detection using the LNA technique

查看全文 作　　者：Ji [1]Wang;Li [1,2]Zhao;Zhihua [3]Sun;Guixia [4]Li;Peihua [1]Niu;DANDi [1]Li;Lianjun [5]Wang;Yi [1]Zhang;Zhishan [4]Feng;Xuejun [1]Ma

机构地区：[1]National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention;[2]Hebei Medical University;[3]Beijing Chaoyang Maternal & Child Health Hospital;[4]Children's Hospital of Hebei Province;[5]Dongcheng Center for Disease Control and Prevention,Beijing

出　　处：《Science China(Life Sciences)》索引2019年第62卷第3期,共3页

基　　金：supported by the National Key Research and Development Plan (2016TFC1202700, 2016YFC1200900);Beijing Municipal Science & Technology Commission Project (D151100002115003);Guangzhou Municipal Science & Technology Commission project (2015B2150820) 摘　　要：Dear Editor,Polymerase chain reaction(PCR)is a molecular diagnostic technique that has been widely used for diagnosing viral diseases.Nested PCR(N-PCR)is a variation on the standard PCR technique that involves two amplification steps,yielding greater sensitivity and specificity.But there are two main disadvantages:the protocol is more complex than conventional PCR,and the risk of cross-contamination is relatively high.One-step nested PCR(OSN-PCR)enables conventional N-PCR to be performed as a closed reaction in a single tube that contains both outer and inner primers.This strategy makes OSN-PCR more rapid than N-PCR,lowers the probability of cross-contamination,and requires a smaller amount of reagents. 关键词：DEAR EDITOR PCR STRATEGY LNA TECHNIQUE

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Development of an innovative one-step nested PCR strategy for virus detection using the LNA technique

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