Direct and single-step sensing of primary ovarian cancers related glycosidases

查看全文 作　　者：Dan [1]Li;Ling [1]Liang;Yawei [1]Tang;Linna [1]Fu;Shehua [1]Xiao;Quan [1]Yuan

机构地区：[1]Molecular Science and Biomedicine Laboratory, Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University

出　　处：《Chinese Chemical Letters》索引2019年第30卷第5期,共4页

基　　金：supported by the National Key R&D Program of China(No.2017YFA0208000);the National Natural Science Foundation of China(No.21675120);Ten Thousand Talents Program for Young Talents,the Foundation for Innovative Research Groups of the National Nature Science Foundation of China(No.21521063);the Start-up Research Fund for Prof.Q.Yuan(Nos.531107050973,531109010053) 摘　　要：High sensitive,accurate detection for tumor-associated overexpressed enzyme activity is highly significant for further understanding enzyme function,discovering potential drugs,and early diagnosis and prevention of diseases.In this work,we developed a facile,direct and single-step detection platform for primary ovarian cancers related glycosidase activity based on the inner filter effect(IFE)between glycosidase catalytic product and black phosphorus quantum dots(BPQDs).Highly fluorescent BPQDs were successfully synthesized from bulk black phosphorus by a simple liquid exfoliation method.Under the catalysis ofβ-galactosidase,p-nitrophenyl-β-D-galactopyranoside(PNPG)was transformed into pnitrophenol(PNP)andβ-D-galactopyranoside.Meanwhile,the absorption of catalytic product PNP greatly overlapped with the excitation and emission spectra of fluorescent BPQDs,leading to the fluorescence quenching of BPQDs with a high quenching efficiency.The proposed sensing strategy provided a low detection limit of 0.76 U/L,which was 1-2 orders of magnitude lower than most unmodified sensing platforms.D-Galactal was selected as the inhibitor forβ-galactosidase to further assess the feasibility of screening potential inhibitors.The fluorescence recovery of BPQDs suggests that the unmodified sensing platform is feasible to discover potential drugs ofβ-galactosidase.Our work paves a general way in the detection of glycosidase activity with fluorescent BPQDs,which can be promising for glycosidase-related disease diagnosis and pathophysiology elucidation. 关键词：Black phosphorus quantum DOTS Fluorescence SENSING Β-GALACTOSIDASE INNER filter effect

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Direct and single-step sensing of primary ovarian cancers related glycosidases

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