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Protective Effect of Angiotensin(1-7) on Silicotic Fibrosis in Rats

查看全文 作　　者：ZHANG Bo [1,2,3]Nan;XU [2]Hong;GAO Xue [4]Min;ZHANG Gui [2,3]Zhen;ZHANG [2]Xin;YANG [2]Fang 高影响力作者 机构地区：[1]School of Public Health, North China University of Science and Technology, Tangshan 063210, Hebei, China;[2]Hebei Key Laboratory for Organ Fibrosis, Medical Research Center, North China University of Science and Technology, Tangshan 063210, Hebei, China;[3]Hebei Key Laboratory for Chronic Diseases, Tangshan Key Laboratory for Clinical and Basic Research on Chronic Diseases, School of Basic Medical Sciences, North China University of Science and Technology, Tangshan 063210, Hebei, China;[4]Basic Medical College, Hebei Medical University, Shijiazhuang 050017, Hebei, China高影响力机构 出　　处：《Biomedical and Environmental Sciences》索引2019年第32卷第6期,共8页高影响力期刊 基　　金：supported by the National Natural Science Foundation of China [grant no.81472953];Natural Science Foundation of Hebei Province [grant no.H2016209170];Graduate Student Innovation Fund of Hebei Province [grant no.2016196];Graduate Student Innovation Fund of North China University of Science and Technology [grant no.2016B10];undergraduate innovative project of North China University of Science and Technology [grant no.X2017354] 摘　　要：Objective Silicosis, caused by inhalation of silica dust, is the most serious occupational disease in China and the aim of present study was to explore the protective effect of Ang (1-7) on silicotic fibrosis and myofibroblast differentiation induced by Ang Ⅱ. Methods HOPE-MED 8050 exposure control apparatus was used to establish the rat silicosis model. Pathological changes and collagen deposition of the lung tissue were examined by H.E. and VG staining, respectively. The localizations of ACE2 and a-smooth muscle actin (a-SMA) in the lung were detected by immunohistochemistry. Expression levels of collagen type Ⅰ, a-SMA, ACE2, and Mas in the lung tissue and fibroblasts were examined by western blot. Levels of ACE2, Ang (1-7), and Ang Ⅱ in serum were determined by ELISA. Co-localization of ACE2 and a-SMA in fibroblasts was detected by immunofluorescenee. Results Ang (1-7) induced pathological changes and enhanced collagen deposition in vivo. Ang (1-7) decreased the expressions of collagen type Ⅰ and a-SMA and increased the expressions of ACE2 and Mas in the silicotic rat lung tissue and fibroblasts stimulated by Ang Ⅱ. Ang (1-7) in creased the levels of ACE2 and Ang (1-7) and decreased the level of Ang Ⅱ in silicotic rat serum. A779 enhanced the protective effect of Ang (1-7) in fibroblasts stimulated by Ang Ⅱ. Conclusion Ang (1-7) exerted protective effect on silicotic fibrosis and myofibroblast differentiation induced by Ang Ⅱ by regulating ACE2-Ang (l-7)-Mas axis. 关 键 词：SILICOSIS ANG Ⅱ MYOFIBROBLASTS ANG (1-7) A779
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