Immunological Evaluation of a Novel Mycobacterium tuberculosis Antigen Rv0674

查看全文 作　　者：XIAO Tong [1]Yang;LIU Hai [1]Can;LI Xiao [1,2]Qin;HUANG Ming [3]Xiang;LI Gui [1]Lian;LI [1]Na;YAN Yu [1,2]Han;LUO [4]Qiao;WANG Xue [5]Zhi;LI Ma [1]Chao;WAN Kang [1]Lin

机构地区：[1]State Key Laboratory for Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;[2]Key Laboratory of Laboratory Medicine, Ministry of Education, College of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China;[3]Clinical laboratory, Fuzhou Pulmonary Hospital, Fuzhou 350008, Fujian, China;[4]The first hospital of Changsha, Changsha 410005, Hunan, China;[5]Key laboratory of Translational Medicine of Guangdong, Center for Tuberculosis Control of Guangdong Province, Guangzhou 510630, Guangdong, China

出　　处：《Biomedical and Environmental Sciences》索引2019年第32卷第6期,共11页

基　　金：National Science and Technology Major Project of China [2018ZX10731301-002] 摘　　要：Objective This study aimed to characterize the diagnostic and vaccine potential of a novel Mycobacterium tuberculosisantigen Rv0674. Methods To evaluate thediagnostic potential and antigenicity of Rv0674, IgG was evaluated using ELISA and interferon (IFN)-γ was done by using ELISpot assay among TB patients and healthy donors. For immunogenicity evaluation, BALB/c mice were immunized with Rv0674. Cytokine production was determined by cytokine release assay using an ELISA kit, and the antibodies were tested using ELISA. Results The results of serum Elisa tests showed that Rv0674 specific immunoglobulin G (IgG) response was higher in TB patients than negative controls. And Rv0674 had good performance in serological test with sensitivity and specificity of 77.1% and 81.1%, respectively. While it shows poor sensitivity and specificity of 26.23% and 79.69% for IFN-γ tests. In BALB/c mice, Rv0674 adjuvant by DDA/PolyI:C could also induce a high level of IFN-γ, interleukin-2 and interleukin-6 as well as a high IgG titer in both high-and low-dose groups indicating that Rv0674 is essential in humoral and cellular immunity. Moreover, the cytokine profile and IgG isotypecharacterized Rv0674 as a Th1/Th2-mixed-type protective immunity with the predominance of Th1 cytokines. Conclusion Rv0674 may be a good potential candidate for the development of TB serological diagnosis and a new TB vaccine. 关键词：ANTIGEN IMMUNOLOGIC diagnosis MYCOBACTERIUM TUBERCULOSIS Rv0674 VACCINE

相关文献

参考文献（42）
引证文献（6）
耦合文献（9）

参考文献(42)

引证文献(6)

耦合文献(9)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Immunological Evaluation of a Novel Mycobacterium tuberculosis Antigen Rv0674

参考文献(42)

引证文献(6)

耦合文献(9)

用户提示