Association of hTcf-4 gene expression and mutation with clinicopathological characteristics of hepatocellular carcinoma

查看全文 作　　者：Ying Jiang Xin-Da Zhou Yin-Kun Liu Xin Wu Xiao-Wu Huang Liver Cancer Institute,Zhong Shah Hospital,Fudan University,Shanghai 200032,China

出　　处：《World Journal of Gastroenterology》索引2002年第8卷第5期,共4页

基　　金：National Natural Science Foundation of China,No.30070743 摘　　要：AIM: Hepatocellular carcinoma(HCC) is a significant healthproblem in China. But the molecular mechanisms of HCCremains unclear. APC/β-Catenin/Tcf signaling pathway, alsoknown as Wnt pathway, plays a critical role in thedevelopment and oncogenesis. As little is known about thealteration of human T-cell transcription factor-4 (hTcf-4) genein HCC, it is of interest to study the expression and mutationof hTcf-4 gene in HCC and the relationship between hTcf-4gene and progression of HCC.METHODS: Reverse transcription-polymerase chain reaction(RT-PCR) method was used to detect the expression of hTcf-4 mRNA in 32 HCC and para-cancerous tissues and 5 normalliver tissues. PCR-single strand conformation polymorphism(PCR-SSCP) method was used to detect the mutation ofhTcf-4 exons 1, 4, 9 and 15 in HCC. The correlation ofexpression and mutation of the hTcf-4 gene withclinicopathological characteristics of HCC was also analyzed.RESULTS: RT-PCR showed that the expression rate of hTcf-4 mRNA in HCC, para-cancerous tissues and normal livertissues was 90.6 %, 71.9 % and 80 %, respectively. Thegene expression level in tumor was 0.71±0.13, much higherthan that in para-cancerous liver 0.29±0.05 and normal liver0.26±0.05 (P<0.001), although there was no significantdifference in gene expression level between para-canceroustissues and normal liver (P>0.05). Furthermore, hTcf-4 geneexpression was closely associated with tumor capsule statusand intrahepatic metastasis of HCC. On SSCP, 2 of 32 casesof HCC (6.25 %) displayed characteristic mutational mobilityshifts in exon 15 of the hTcf-4 gene. No abnormal shiftingbands were observed in para-cancerous tissues.CONCLUSION: The high expression level of hTcf-4 in HCC,especially in tumors with metastasis, suggests that the over-expression of hTcf-4 gene may be closely associated withdevelopment and progression of HCC, but the mutation ofthis gene seemed to play less important role in this respect. 关键词：肝癌临床病理学基因突变 RT-PCR hTcf-4基因

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Association of hTcf-4 gene expression and mutation with clinicopathological characteristics of hepatocellular carcinoma

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