Analytical methodologies for determination of methotrexate and its metabolites in pharmaceutical,biological and environmental samples

查看全文 作　　者：Forough [1,2,3]Karami;Sara [1]Ranjbar;Younes [1,4]Ghasemi;Manica [1]Negahdaripour

机构地区：[1]Pharmaceutical Sciences Research Center,Shiraz University of Medical Sciences,Shiraz,Iran;[2]Central Research Laboratory,School of Medicine,Shiraz University of Medical Sciences,Shiraz,Iran;[3]Chemistry Department,Yasouj University,Yasouj,Iran;[4]Department of Pharmaceutical Biotechnology,School of Pharmacy,Shiraz University of Medical Sciences,Shiraz,Iran

出　　处：《Journal of Pharmaceutical Analysis》索引2019年第9卷第6期,共19页

基　　金：support of the Vice-Chancellor for Research,Shiraz University of Medical Sciences,Iran(project No.97-01-36-18308) 摘　　要：Methotrexate(MTX)is a folate antagonist drug used for several diseases,such as cancers,various malignancies,rheumatoid arthritis(RA)and inflammatory bowel disease.Due to its structural features,including the presence of two carboxylic acid groups and its low native fluorescence,there are some challenges to develop analytical methods for its determination.MTX is metabolized to 7-hydroxymethotrexate(7-OH-MTX),2,4-diamino-N10-methylpteroic acid(DAMPA),and the active MTX polyglutamates(MTXPGs)in the liver,intestine,and red blood cells(RBCs),respectively.Additionally,the drug has a narrow therapeutic range;hence,its therapeutic drug monitoring(TDM)is necessary to regulate the pharmacokinetics of the drug and to decrease the risk of toxicity.Due to environmental toxicity of MTX;its sensitive,fast and low cost determination in workplace environments is of great interest.A large number of methodologies including high performance liquid chromatography equipped with UVevisible,fluorescence,or electrochemical detection,liquid chromatography-mass spectroscopy,capillary electrophoresis,UVevisible spectrophotometry,and electrochemical methods have been developed for the quantitation of MTX and its metabolites in pharmaceutical,biological,and environmental samples.This paper will attempt to review several published methodologies and the instrumental conditions,which have been applied to measure MTX and its metabolites within the last decade. 关键词：METHOTREXATE Antifolate ANTICANCER Quantification High performance liquid chromatography

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Analytical methodologies for determination of methotrexate and its metabolites in pharmaceutical,biological and environmental samples

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