The ruminal bacterial community in lactating dairy cows has limited variation on a day-to-day basis

查看全文 作　　者：Joseph [1]H.Skarlupka;Maria [2,3]E.Kamenetsky;Kelsea [4]A.Jewell;Garret [1]Suen 机构地区：[1]Department of Bacteriology,University of Wisconsin-Madison,5159 MSB,1550 Linden Drive,Madison,WI 53706,USA;[2]Department of Population Health Sciences,University of Wisconsin-Madison,Madison 53726,USA;[3]College of Agricultural and Life Sciences Statistical Consulting Lab,University of Wisconsin-Madison,Madison 53706,USA;[4]Department of Biology,Centralia College,Centralia,WA 98531,USA. 出　　处：《Journal of Animal Science and Biotechnology》索引2019年第10卷第4期,共5页 基　　金：supported by Agriculture and Food Research Initiative Competitive Grant nos.2013–67015-21348 and 2015–67015-23246 from the USDA National Institute of Food and Agriculture 摘　　要：Dairy cows rely on a complex ruminal microbiota to digest their host-indigestible feed. Our ability to characterize this microbiota has advanced significantly due to developments in next-generation sequencing. However, efforts to sample the rumen, which typical y involves removing digesta directly from the rumen via a cannula, intubation, or rumenocentesis, is costly and labor intensive. As a result, the majority of studies characterizing the rumen microbiota are conducted on samples col ected at a single time point. Currently, it is unknown whether there is significant day-to-day variation in the rumen microbiota, a factor that could strongly influence conclusion drawn from studies that sample at a single time point. To address this, we examined day-to-day changes in the ruminal microbiota of lactating dairy cows using next-generation sequencing to determine if single-day sampling is representative of sampling across 3 consecutive days. We sequenced single-day solid and liquid fractions of ruminal digesta col ected over 3 consecutive days from 12 cannulated dairy cows during the early, middle, and late stages of a single lactation cycle using the V4 region of the bacterial 16 S r RNA gene. We then generated 97% similarity operational taxonomic units(OTUs) from these sequences and showed that any of the individual samples from a given 3-day sampling period is equivalent to the mean OTUs determined from the combined 3-d data set. This finding was consistent for both solid and liquid fractions of the rumen,and we thus conclude that there is limited day-to-day variability in the rumen microbiota. 关 键 词：Bacterial community Next-generation SEQUENCING RUMEN MICROBIOTA