Expression Profile and Function Analysis of Long Non-coding RNAs in the Infection of Coxsackievirus B3

查看全文 作　　者：Lei [1]Tong;Ye [2]Qiu;Hui [1]Wang;Yunyue [1]Qu;Yuanbo [2]Zhao;Lexun [1]Lin;Yan [3]Wang;Weizhen [1]Xu;Wenran [3]Zhao;Hongyan [2]He;Guangze [4]Zhao;Mary [4]HZhang;Decheng [4]Yang;Xingyi [2]Ge;Zhaohua [1]Zhong

机构地区：[1]Department of Microbiology,Harbin Medical University,Harbin 150081,China;[2]College of Biology,Hunan University,Changsha 410012,China;[3]Department of Cell Biology,Harbin Medical University,Harbin 150081,China;[4]Department of Pathology and Laboratory Medicine,University of British Columbia,Vancouver,BC V6Z 1Y6,Canada

出　　处：《Virologica Sinica》索引2019年第34卷第6期,共13页

基　　金：supported by the National Natural Science Foundation of China (81101234 to Lei Tong; 81571999, 81871652 to Zhaohua Zhong; 31470260 to Xingyi Ge; 81672007 to Wenran Zhao; 81772188 to Yan Wang);the Foundation of Heilongjiang Provincial Postdoctor of China (LBH-Z11076 to Lei Tong);the China Postdoctoral Science Foundation (2015M580269 to Lexun Lin);the Research Foundation of Education Bureau of Heilongjiang Province (12511176 to Lei Tong);the Hu-Xiang Youth Talents Scholar Program of Hunan Province (2017RS3017 to Xingyi Ge);Health and Family Planning Commission of Heilongjiang Province (2016-165 to Lexun Lin);the Provincial Natural Science Foundation of Hunan Province (Grant Number 2019JJ50035 to Ye Qiu);the Fundamental Research Funds for the Central Universities of China (Grant Number 531107051162 to Ye Qiu) 摘　　要：The roles of IncRNAs in the infection of enteroviruses have been barely demonstrated.In this study,we used coxsackievirus B3(CVB3),a typical enterovirus,as a model to investigate the expression profiles and functional roles of IncRNAs in enterovirus infection.We profiled IncRNAs and mRNA expression in CVB3-infected HeLa cells by IncRNA-mRNA integrated microarrays.As a result,700 differentially expressed IncRNAs(431 up-regulated and 269 down-regulated)and 665 differentially expressed mRNAs(299 up-regulated and 366 down-regulated)were identified in CVB3 infection.Then we performed IncRNA-mRNA integrated pathway analysis to identify potential functional impacts of the differentially expressed mRNAs,in which IncRNA-mRNA correlation network was built.According to IncRNA-mRNA correlation,we found that XLOC-001188,an IncRNA down-regulated in CVB3 infection,was negatively correlated with NFAT5 mRNA,an anti-CVB3 gene reported previously.This interaction was supported by qPCR detection following siRNA-mediated knockdown of XLOC-001188,which showed an increase of NFAT5 mRNA and a reduction of CVB3 genomic RNA.In addition,we observed that four most significantly altered IncRNAs,SNHG11,RP11-145F16.2,RP11-1023L17.1 and RP11-1021N1.2 share several common correlated genes critical for CVB3 infection,such as BRE and IRF2BP1.In all,our studies reveal the alteration of IncRNA expression in CVB3 infection and its potential influence on CVB3 replication,providing useful information for future studies of enterovirus infection. 关键词：Coxsackievirus B3(CVB3) IncRNA-mRNA correlation network Long non-coding RNA(IncRNA) XLOC-001188 NFAT5

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Expression Profile and Function Analysis of Long Non-coding RNAs in the Infection of Coxsackievirus B3

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