维普中文期刊产品整合服务

Simultaneous, hybrid single-molecule method by optical tweezers and fluorescence

查看全文 作　　者：Guoteng [1,2]Ma;Chunguang [1,2]Hu;Shuai [1,2]Li;Xiaoqin [1,2]Gao;Hongbin [1,3]Li;Xiaotang [1,2]Hu 高影响力作者 机构地区：[1]State Key Laboratory of Precision Measuring Technology and Instruments,Tianjin University,300072,China;[2]Nanchang Institute for Microtechnology of Tianjin University,Tianjin 300072,China;[3]Department of Chemistry,University of British Columbia,Vancouver,BC V6T 1Z1,Canada高影响力机构 出　　处：《Nanotechnology and Precision Engineering》索引2019年第2卷第4期,共12页高影响力期刊 基　　金：supported by the National Key Research and Development Program of China [grant numbers 2016YFB1102203, 2017YFF0107003] 摘　　要：As studies on life sciences progress toward the single-molecule level,new experiments have put forward more requirements for simultaneously displaying the mechanical properties and conformational changes of biomolecules.Optical tweezers and fluorescence microscopy have been combined to solve this problem.The combination of instruments forms a new generation of hybrid single-molecule technology that breaks through the limitations of traditional biochemical analysis.Powerfulmanipulation and fluorescence visualization have beenwidely used,and these techniques provide new possibilities for studying complex biochemical reactions at the singlemolecule level.This paper explains the features of this combined technique,including the application characteristics of single-trap and dual-traps,the anti-bleaching method,and optical tweezers combined with epifluorescence,confocal fluorescence,total internal reflection fluorescence,and other fluorescence methods.Using typical experiments,we analyze technical solutions and explain the factors and principles that instrument designers should consider.This review aims to give an introduction to this novel fusion technology process and describe important biological results. 关 键 词：SINGLE-MOLECULE Optical tweezers Confocal fluorescence FRET TIRF
相关文献

参考文献(93)

引证文献(1)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费