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Family-level diversity of extracellular proteases of sedimentary bacteria from the South China Sea

查看全文 作　　者：Jinyu [1,4]Yang;Yangyang [1]Feng;Xiulan [1]Chen;Binbin [1]Xie;Yuzhong [1,2,3]Zhang;Mei [1]Shi;Xiying [1]Zhang 高影响力作者 机构地区：[1]State Key Laboratory of Microbial Technology,Marine Biotechnology Research Center,Shandong University,Qingdao 266237,China;[2]Laboratory for Marine Biology and Biotechnology,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266237,China;[3]College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China;[4]Institute of Agro-Food Science and Technology,Shandong Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing Technology of Shandong Province/Key Laboratory of Novel Food Resources Processing,Ministry of Agriculture and Rural Affairs,Jinan 250100,China高影响力机构 出　　处：《Acta Oceanologica Sinica》索引2019年第38卷第12期,共11页高影响力期刊 基　　金：The AoShan Talents Cultivation Program supported by Qingdao National Laboratory for Marine Science and Technology under contract No.2017ASTCP-OS14;the National Natural Science Foundation of China under contract Nos 31670063,31670497 and 31870052;the Taishan Scholars Program of Shandong Province under contract No.2009TS079;the Science and Technology Basic Resources Investigation Program of China under contract No.2017FY100804 摘　　要：Protease-producing bacteria and their extracellular proteases are key players in degrading organic nitrogen to drive marine nitrogen cycling and yet knowledge on both of them is still very limited. This study screened protease-producing bacteria from the South China Sea sediments and analyzed the diversity of their extracellular proteases at the family level through N-terminal amino acid sequencing. Results of the 16 S rRNA gene sequence analysis showed that all screened protease-producing bacteria belonged to the class Gammaproteobacteria and most of them were affiliated with different genera within the orders Alteromonadales and Vibrionales. The Nterminal amino acid sequence analysis for fourteen extracellular proteases from fourteen screened bacterial strains revealed that all these proteases belonged to the M4 family of metalloproteases or the S8 family of serine proteases. This study presents new details on taxa of marine sedimentary protease-producing bacteria and types of their extracellular proteases, which will help to comprehensively understand the process and mechanism of the microbial enzymatic degradation of marine sedimentary organic nitrogen. 关 键 词：protease-producing bacteria DIVERSITY extracellular proteases protease families N-terminal amino acid sequencing South China Sea
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