脐带间充质干细胞治疗SUI鼠模及干细胞标记磁共振示踪检测体内的实验效果

查看全文 作　　者：[1,2]罗新;[2,3]季菲;[2]蓝剑发;[3]朱元方;[2]宋红;[2]石海燕;[2]杨斌见;[1,2]蒋学峰

机构地区：[1]Dept.Of Ob&Gyn,The 1st Affelieted Hospital of Jinan University;[2]The 1st Clinic School of Jinan University;[3]Jinan University Affiliated Baoan District Maternal and Child Health Care Hospital

出　　处：《Chinese Journal Of Plastic and Reconstructive Surgery》索引2019年第1卷第2期,共12页

摘　　要：Objective To assess the effect of intra-sphincteric injections of human umbilical cord mesenchymal stem cells(HUMSCs)on leak point pressure(LPP)changes in an animal model of stress urinary incontinence(SUI).Meanwhile,to investigate in vivo MRI tracking HUMSCs in SUI rats using a clinically available paramagnetic contrast agent(Gd-DTPA)and commercially available effentence transfection reagents..Materials and Methods HUMSCs were dual labeled with Gd-DTPA and PKH26,the labeling efﬁciency and longevity of Gd-DTPA maintenance were measured and cell viability and proliferation were assessed.39 female Sprague–Dawley SUI rats.12 normal rats and 12 SUI rats received periurethral injection of PBS and 12 SUI rats were given periurethral injection of dual labeled HUMSCs.3 SUI rat sreceived periurethral injection of u nlabeled HUMSCs.Six weeks after injection,LPP was undertaken in animals.All rats were sacriﬁced and frozen urethra sections were submitted to pathology and immunohistochemistry assessment.Results The labeling efﬁciency of Gd-DTPA was up to 80%,the labeling procedure did not influence cell viability and proliferation.The signal intensity on T1-weighted imaging and T1 values of labeled cells were signiﬁcantly higher than those of unlabeled cells.In vitro,differentiated HUMSCs expressed myosin heavy chain(MHC)and desmin,markers of striated muscles.In vivo,immunohistochemistry of rat urethras revealed dual labeled HUMSCs in situ and at the injection site.LPP was signiﬁcantly improved in animals injected with HUMSCs.Atrophic urethras with implanted HUMSCs were positively stained for MHC and desmin.The distribution and migration of labeled cells could be tracked by MRI more than 14 days after t ransplantation.Conclusion HUMSCs have the ability to differentiate striated muscles,as demonstrated by MHC and desmin expression.Periurethral injection of HUMSCs in an animal model of SUI restored the damaged external urethral sphincter and signiﬁcantly improved LPP.MRI can track Gd-DTPA–labled HUMSCs in an animal model of SUI in vivo. 关键词：Human Umbilical Cord Mesenchymal Stromal Cells stress urinary incontinence leak point pressure Effentence transfection reagents PKH26 GD-DTPA MHC DESMIN PGP9.5 MRI.

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脐带间充质干细胞治疗SUI鼠模及干细胞标记磁共振示踪检测体内的实验效果

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