Synchrotron Radiation-Based Three-Dimensional Visualization of Angioarchitectural Remodeling in Hippocampus of Epileptic Rats

查看全文 作　　者：Pan [1]Gu;Zi-Hao [1]Xu;Yu-Ze [1]Cao;Sheng-Hui [2]Liao;Qian-Fang [1]Deng;Xian-Zhen [3]Yin;Zhuo-Lu [4]Wang;Zhuo-Hui [1]Chen;Xin-Hang [1]Hu;Hui [1]Wang;Li-Zhi [1]Li;Shi-Xin [1]Liu;Hul [1]Ding;Shu-Peng [1]Shi;Hong-Lei [1]Li;Ti-Qiao [5]Xiao;Bo [1]Xiao;Meng-Qi [1]Zhang

机构地区：[1]Department of Neurology,Xiangya Hospital,Central South University,Changsha 410008,China;[2]School of Information Science and Engineering,Central South University,Changsha 410008,China;[3]State Key Laboratory of Drug Research,CAS Key Laboratory of Receptor Research,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China;[4]Department of Breast Surgery,Hunan Provincial Maternal and Child Health Care Hospital,Changsha 410008,China;[5]Shanghai Synchrotron Radiation Facility,Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai 201800,China

出　　处：《Neuroscience Bulletin》索引2020年第36卷第4期,共13页

基　　金：the BL13W1 beamline of the Shanghai Synchrotron Radiation Facility(SSRF)in China and was supported by Key Research Project of the Ministry of Science and Technology of China(2016YFC0904400);the National Natural Science Foundation of China(81501025&81671299);the Natural Science Foundation of Hunan Province(2016JJ3174);the Science and Technology Department Funds of Hunan Province Key Project(2016JC2057). 摘　　要：Characterizing the three-dimensional (3D) morphological alterations of microvessels under both normal and seizure conditions is crucial for a better understanding of epilepsy. However, conventional imaging techniques cannot detect microvessels on micron/sub-micron scales without angiography. In this study, synchrotron radiation(SR)-based X-ray in-line phase-contrast imaging (ILPCI)and quantitative 3D characterization were used to acquire high-resolution, high-contrast images of rat brain tissue under both normal and seizure conditions. The number of blood microvessels was markedly increased on days 1 and 14, but decreased on day 60 after seizures. The surface area, diameter distribution, mean tortuosity, and number of bifurcations and network segments also showed similar trends. These pathological changes were confirmed by histological tests. Thus, SR-based ILPCI provides systematic and detailed views of cerebrovascular anatomy at the micron level without using contrast-enhancing agents. This holds considerable promise for better diagnosis and understanding of the pathogenesis and development of epilepsy. 关键词：EPILEPSY SYNCHROTRON radiation 3D ANGIOARCHITECTURE Blood VESSEL REMODELING

相关文献

参考文献（78）
引证文献（3）
耦合文献（49）

参考文献(78)

引证文献(3)

耦合文献(49)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Synchrotron Radiation-Based Three-Dimensional Visualization of Angioarchitectural Remodeling in Hippocampus of Epileptic Rats

参考文献(78)

引证文献(3)

耦合文献(49)

用户提示