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Enzyme-digested Colla Corii Asini(E’jiao) prevents hydrogen peroxide-induced cell death and accelerates amyloid beta clearance in neuronal-like PC12 cells

查看全文 作　　者：Li [1]Xiao;Feng [2]Liao;Ryoji [3]Ide;Tetsuro [4]Horie;Yumei [2]Fan;Chikako [3]Saiki;Nobuhiko [5]Miwa 高影响力作者 机构地区：[1]Department of Pharmacology,The Nippon Dental University,School of Life Dentistry at Tokyo,Tokyo,Japan;[2]National Engineering Research Center for Gelatin-based Traditional Chinese Medicine,Dong-E-E-Jiao Co.Ltd.,Liaocheng,Shandong Province,China;[3]Department of Physiology,The Nippon Dental University,School of Life Dentistry at Tokyo,Tokyo,Japan;[4]Research Center,The Nippon Dental University,School of Life Dentistry at Tokyo,Tokyo,Japan;[5]Department of Life Sciences,Prefectural University of Hiroshima,Hiroshima,Japan高影响力机构 出　　处：《Neural Regeneration Research》索引2020年第15卷第12期,共8页高影响力期刊 基　　金：supported in part by the Japan Society for the Promotion of Science(JSPS)Grant-in-Aid for Scientific Research,No.17K11813(to LX);Tai Shan Industrial Experts Program of China No.TSCY20170233(to FL)。 摘　　要：As an aging-associated degenerative disease,Alzheimer’s disease is characterized by the deposition of amyloid beta(Aβ),oxidative stress,inflammation,dysfunction and loss of cholinergic neurons.Colla Corii Asini(CCA)is a traditional Chinese medicine which has been used for feebleness-related diseases and anti-aging.CCA might delay aging-induced degenerative changes in neurons.In the present study,we evaluated antioxidant activity,cytoprotective effects,and Aβremovability of enzyme-digested Colla Corii Asini(CCAD).Oxygen radical absorbance capacity(ORAC)activity assay showed that,as compared to gelatins from the skin of porcine,bovine and cold water fish,CCA exhibited the highest ORAC activity.The ORAC activity of CCA and CCAD was increased gradually by the length of time in storage.Ultrastructure analysis by scanning electron microscopy showed that among CCA manufactured in 2008,2013,2017 and gelatin from cold water fish skin,CCA manufactured in 2008 presented the smoothest surface structure.We further tested the protective effects of CCAD(manufactured in 2008)and enzyme-digested gelatin from cold water fish skin(FGD)on hydrogen peroxide(H2O2)-induced cell death in nerve growth factor-differentiated neuronal-like PC12 cells.Presto blue assay showed that both FGD and CCAD at 0.5 mg/m L increased cell viability in H2O2-treated neuronal-like PC12 cells.The protection of CCAD was significantly superior to that of FGD.Acetylcholinesterase(Ach E)assay showed that both FGD and CCAD inhibited Ach E activity in nerve growth factor-differentiated neuronal-like PC12 cells to 89.1%and 74.5%of that in non-treated cells,respectively.The data suggest that CCAD might be able to increase the neurotransmitter acetylcholine.Although CCAD inhibited Ach E activity in neuronal-like PC12 cells,CCAD prevented H2O2-induced abnormal deterioration of Ach E.ELISA and neprilysin activity assay results indicated that CCAD reduced amyloid beta accumulation and increased neprilysin activity in Aβ1–42-treated neuronal-like PC12 cells,suggesting that CCAD can enhance Aβclearance.Our results suggest that CCA might be useful for preventing and treating Alzheimer’s disease. 关 键 词：acetylcholinesterase activity Alzheimer’s disease amyloid beta clearance antioxidant Colla Corii Asini collagen NEUROPROTECTION PC12 cells
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