维普中文期刊产品整合服务

Salt-tolerant mechanism of marine Aspergillus niger cellulase cocktail and improvement of its activity

查看全文 作　　者：Linian [1]Cai;Shengnan [1]Xu;Tao [2]Lu;Dongqiang [1]Lin;Shanjing [1]Yao 高影响力作者 机构地区：[1]Key Laboratory of Biomass Chemical Engineering of Ministry of Education,College of Chemical and Biological Engineering,Zhejiang University,Hangzhou 310027,China;[2]College of Environment,Zhejiang University of Technology,Hangzhou 310014,China高影响力机构 出　　处：《Chinese Journal of Chemical Engineering》索引2020年第28卷第4期,共9页高影响力期刊 基　　金：supported by National Natural Science Foundation of China(21576233,21878263);Fundamental Research Funds for the Central Universities。 摘　　要：The cellulase cocktail produced by marine Aspergillus niger exhibits a property of salt-tolerance,which is of great potential in cellulose degradation in high salt environment.In order to explain the mechanism on the salttolerance of the cellulase cocktail produced by marine A.niger,six cellulase components(AnCel6,AnCel7A,AnCel7B,AnEGL,AnBGL1 and AnBGL2)were obtained by directed expression.Studies on their enzymatic properties revealed that oneβ-glucosidase(AnBGL2)and one endoglucanase(AnEGL)exhibited an outstanding salttolerant property,and one cellobiohydrolase(AnCel7B)exhibited a certain salt-tolerant property.Subsequent study revealed that the salt-tolerant An EGL and AnCel7B endowed the cellulase cocktail with stronger salttolerant property,while the salt-tolerant An BGL2 had no positive effect.Moreover,after overexpression of AnCel6,AnCel7A,AnCel7B and AnEGL,the activity of cellulase cocktail increased by 80%,70%,63%and 68%,respectively.However,the activity of cellulase cocktail was not improved after overexpression of AnBGL1 and AnBGL2.After mixed-strain fermentation with cellobiohydrolase recombinants(cel6 a,cel7a and cel7b recombinants)and endoglucanase recombinant(egl recombinant),the the activity of cellulase cocktail increased by 114%,102%and91%,respectively. 关 键 词：Marine Aspergillus niger Cellulase component Directed expression SALT-TOLERANCE Cellulase activity
相关文献

参考文献(40)

引证文献(2)

耦合文献(63)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费