Gastrodin protects H9c2 cardiomyocytes against oxidative injury by ameliorating imbalanced mitochondrial dynamics and mitochondrial dysfunction

查看全文 作　　者：Qiao-qiao [1]Cheng;Yu-wei [1]Wan;Wei-min [2]Yang;Meng-hua [3]Tian;Yu-chuan [3]Wang;Hai-yan [3]He;Wei-dong [1]Zhang;Xuan [1]Liu

机构地区：[1]Institute of Interdisciplinary Integrative Biomedical Research,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;[2]School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical University,Kunming 650500,China;[3]Zhaotong Institute of Tianma,Zhaotong 657000,China

出　　处：《Acta Pharmacologica Sinica》索引2020年第41卷第10期,共14页

基　　金：supported by the Chen-Kai-Xian Academician Workstation in Yunnan Province(No.2017IC041);National Natural Science Foundation of China(Nos.81873056 and 81872496);the Open Project of the State Key Laboratory of Innovative Natural Medicine and Traditional Chinese Medicine Injections(No.QFSKL2018006). 摘　　要：Gastrodin(GAS)is the main bioactive component of Tianma,a traditional Chinese medicine widely used to treat neurological disorders as well as cardio-and cerebrovascular diseases.In the present study,the protective effects of GAS on H9c2 cells against ischemia–reperfusion(IR)-like injury were found to be related to decreasing of oxidative stress.Furthermore,GAS could protect H9c2 cells against oxidative injury induced by H2+O2+.Pretreatment of GAS at 20,50,and 100μM for 4 h significantly ameliorated the decrease in cell viability and increase in apoptosis of H9c2 cells treated with 400μM H2+O2+for 3 h.Furthermore,we showed that H2+O2+treatment induced fragmentation of mitochondria and significant reduction in networks,footprint,and tubular length of mitochondria;H2+O2+treatment strongly inhibited mitochondrial respiration;H2+O2+treatment induced a decrease in the expression of mitochondrial fusion factors Mfn2 and Opa1,and increase in the expression of mitochondrial fission factor Fis1.All these alterations in H2+O2+-treated H9c2 cells could be ameliorated by GAS pretreatment.Moreover,we revealed that GAS pretreatment enhanced the nuclear translocation of Nrf2 under H2+O2+treatment.Knockdown of Nrf2 expression abolished the protective effects of GAS on H2+O2+-treated H9c2 cells.Our results suggest that GAS may protect H9c2 cardiomycytes against oxidative injury via increasing the nuclear translocation of Nrf2,regulating mitochondrial dynamics,and maintaining the structure and functions of mitochondria. 关键词：GASTRODIN Tianma H9c2 cardiomycytes oxidative injury NRF2 mitochondrial dynamics mitochondrial respiration

相关文献

参考文献（61）
引证文献（15）
耦合文献（38）

参考文献(61)

引证文献(15)

耦合文献(38)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Gastrodin protects H9c2 cardiomyocytes against oxidative injury by ameliorating imbalanced mitochondrial dynamics and mitochondrial dysfunction

参考文献(61)

引证文献(15)

耦合文献(38)

用户提示