Cryogenic 3D printing of dual-delivery scaffolds for improved bone regeneration with enhanced vascularization

查看全文 作　　者：Chong [1]Wang;Jiahui [2]Lai;Kai [3]Li;Shaokui [1]Zhu;Bingheng [1]Lu;Jia [4]Liu;Yujin [4]Tang;Yen [5]Wei

机构地区：[1]School of Mechanical Engineering,Dongguan University of Technology,Songshan Lake,Dongguan,Guangdong,PR China;[2]Department of Mechanical Engineering,The University of Hong Kong,Hong Kong SAR,PR China;[3]Department of Orthopedics,The Third Affiliated Hospital of Southern Medical University,Guangzhou,Guangdong,PR China;[4]Department of Orthopaedics,Affiliated Hospital of Youjiang Medical University for Nationalities,Baise,Guangxi,PR China;[5]Department of Chemistry,Tsinghua University,Beijing,PR China

出　　处：《Bioactive Materials》索引2021年第6卷第1期,共9页

基　　金：Dongguan University of Technology(KCYCXPT201603,TDYB2019003);Department of Education of Guangdong Province,China(2016KQNCX168);Natural Science Foundation of Guangdong Province,China(2018A0303130019);Natural Science Foundation of China(81772428,81801859);Shenzhen Basic Research Project(JCYJ20180305125254860);J.Liu and Y.Tang were supported by Guangxi Science and Technology Program,China(2018GXNSFAA294116);Guangxi Science and Technology Program,China(2018GXNSFAA138074);Scientific Research Project of High-Level Talents in the affiliated Hospital of Youjiang Medical College for Nationalities,China(R20196306). 摘　　要：Three-dimensional(3D)printing has been increasingly employed to produce advanced bone tissue engineering scaffolds with biomimetic structures and matched mechanical strengths,in order to induce improved bone regeneration in defects with a critical size.Given that the successful bone regeneration requires both excellent osteogenesis and vascularization,endowing scaffolds with both strong bone forming ability and favorable angiogenic potential would be highly desirable to induce improved bone regeneration with required vascularization.In this investigation,customized bone tissue engineering scaffolds with balanced osteoconductivity/osteoinductivity were produced via cryogenic 3D printing ofβ-tricalcium phosphate and osteogenic peptide(OP)containing water/poly(lactic-co-glycolic acid)/dichloromethane emulsion inks.The fabricated scaffolds had a hierarchically porous structure and were mechanically comparable to human cancellous bone.Angiogenic peptide(AP)containing collagen I hydrogel was then coated on scaffold surface to further provide scaffolds with angiogenic capability.A sequential release with a quick AP release and a slow but sustained OP release was obtained for the scaffolds.Both rat endothelial cells(ECs)and rat bone marrow derived mesenchymal stem cells(MSCs)showed high viability on scaffolds.Improved in vitro migration and angiogenesis of ECs were obtained for scaffolds delivered with AP while enhanced osteogenic differentiation was observed in scaffolds containing OP.The in vivo results showed that,toward scaffolds containing both AP and OP,the quick release of AP induced obvious angiogenesis in vivo,while the sustained OP release significantly improved the new bone formation.This study provides a facile method to produce dual-delivery scaffolds to achieve multiple functions. 关键词：Cryogenic 3D printing Dual-delivery OSTEOGENESIS ANGIOGENESIS Bone regeneration

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Cryogenic 3D printing of dual-delivery scaffolds for improved bone regeneration with enhanced vascularization

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