Development of an antimicrobial peptide-loaded mineralized collagen bone scaffold for infective bone defect repair

查看全文 作　　者：Yuzhu [1,2]He;Yahui [1,2,3]Jin;Xiaoxia [2]Ying;Qiong [4]Wu;Shenglian [1]Yao;Yuanyuan [1,2]Li;Huiying [2]Liu;Guowu [2]Ma;Xiumei [1]Wang

机构地区：[1]State Key Laboratory of New Ceramics and Fine Processing,School of Materials Science and Engineering,Tsinghua University,Zhongguancun Street,Haidian District,Beijing 100084,China;[2]Department of Oral Prosthodontics,School of Stomatology,Dalian Medical University,Lvshun South Road,Lushunkou District,Dalian 116044,China;[3]Department of Stomatology,Zhejiang Provincial Hospital of Chinese Medicine,The 9th Street,Economic and Technological Development Zone,Hangzhou 310018,China;[4]School of Life Sciences,Tsinghua University,Zhongguancun Street,Haidian District,Beijing 100084,China

出　　处：《Regenerative Biomaterials》索引2020年第7卷第5期,共11页

基　　金：sponsored by the National Natural Science Foundation of China(Grant Nos 31771056,81671827,51572144,61571077,61871068);the National Key R&D Program of China(No.2018YFB0704304). 摘　　要：The repair of infective bone defects is a great challenge in clinical work.It is of vital importance to develop a kind of bone scaffold with good osteogenic properties and long-term antibacterial activity for local anti-infection and bone regeneration.A porous mineralized collagen(MC)scaffold containing poly(D,L-lactide-co-glycolic acid)(PLGA)microspheres loaded with two antibacterial synthetic peptides,Pac-525 or KSL-W was developed and characterized via scanning electron microscopy(SEM),porosity measurement,swelling and mechanical tests.The results showed that the MC scaffold embedded with smooth and compact PLGA microspheres had a positive effect on cell growth and also had antibacterial properties.Through toxicity analysis,cell morphology and proliferation analysis and alkaline phosphatase evaluation,the antibacterial scaffolds showed excellent biocompatibility and osteogenic activity.The antibacterial property evaluated with Staphylococcus aureus and Escherichia coli suggested that the sustained release of Pac-525 or KSL-W from the scaffolds could inhibit the bacterial growth aforementioned in the long term.Our results suggest that the antimicrobial peptides-loaded MC bone scaffold has good antibacterial and osteogenic activities,thus providing a great promise for the treatment of infective bone defects. 关键词：antibacterial peptides PLGA microspheres mineralized collagen scaffold osteogenic differentiation

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Development of an antimicrobial peptide-loaded mineralized collagen bone scaffold for infective bone defect repair

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