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Efficient Micropropagation of Chestnut Hybrids(Castanea spp.)Using Modified Woody Plant Medium and Zeatin Riboside

查看全文 作　　者：Guoqing [1]Song;Qiuxia [1]Chen;Pete [1]Callow;Mario [2]Mandujano;Xiaoyan [1,3]Han;Beatriz [4]Cuenca;Gregory [2]Bonito;Carmen Medina-[2]Mora;Dennis Wayne [2]Fulbright;Daniel [5]EGuyer 高影响力作者 机构地区：[1]Plant Biotechnology Resource and Outreach Center,Department of Horticulture,Michigan State University,East Lansing,MI 48824,United States;[2]Department of Plant,Soil and Microbial Sciences,Michigan State University,East Lansing,MI 48824,United States;[3]Beijing Botanical Garden,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China;[4]TRAGSA,Vivero de Ourense,Ctra.Maceda-Valdrey km 2,32700 Maceda,Ourense,Spain;[5]Department of Biosystems&Agricultural Engineering,Michigan State University,East Lansing,MI 48824,United States高影响力机构 出　　处：《Horticultural Plant Journal》索引2021年第7卷第2期,共7页高影响力期刊 基　　金：the Michigan State University-Ernie and Mabel Rogers Endowment. 摘　　要：The chestnuts genus(Castanea spp.)is comprised of economically important trees native to the Northern hemisphere that are used as food and hardwood timber.Here,a very efficient method for micropropagation of European×Japanese chestnut hybrids(Castanea sativa×C.crenata)is described.Woody Plant Medium was used as the basal medium.In vitro shoots of four rootstock cultivars were micropropagated without shoot-tip necrosis on multiplication medium containing 5.7 or 11.4μmol·L^(−1)zeatin riboside,and were rooted on rooting medium containing 2.46μmol·L^(−1)indolebutyric acid.Monthly shootmultiplication rates for each cultivarwere 2–5 folds.In vitro rooting percentages for four cultivars were 87%for‘Maraval’,67%for‘Marigoule’,93%for‘Marsol’,and 97%for‘Précoce Migoule’.Within a 5 week period,80%–95%of rooted shoots were successfully acclimated under high humidity conditions after they were planted in either soil or rockwool. 关 键 词：Castanea spp. MICROPROPAGATION shoot multiplication ROOTING tissue culture zeatin riboside ACCLIMATION
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