Impact Factor:1.90 Total flavonoids from Saussurea involucrata attenuate inflammation in lipopolysaccharide-stimulated RAW264.7 macrophages via modulating p65,c-Jun,and IRF3 signaling pathways

查看全文 作　　者：Li-Shan [1]Yan;Li [1]Wang;Brian Chi-Yan [2]Cheng;Yu [1]Ding;Jing [1]Kong;Qing Gao [3]Wang;Xiu-Qiong [4]Fu;Shuo-Feng [1]Zhang;Gan [1]Luo;Yi [1]Zhang

机构地区：[1]School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing,China;[2]College of Professional and Continuing Education,Hong Kong Polytechnic University,Hong Kong,China;[3]The First Affiliated Hospital,Guangxi University of Chinese Medicine,Guangxi,China;[4]Centre for Cancer and Inflammation Research,School of Chinese Medicine,Hong Kong Baptist University,Hong Kong,China

出　　处：《Asian Pacific Journal of Tropical Biomedicine》索引2021年第11卷第6期,共12页

基　　金：This work was supported by the National Natural Science Foundation of China(Grant Number 81803793 and 82003957);the Young Scientist Program by Beijing University of Chinese Medicine,and the Fundamental Research Funds for the Central Universities(Grant Number 2018-JYBZZ-XJSJJ008). 摘　　要：Objective:To investigate the anti-inflammatory effects of the total flavonoids from Saussurea involucrata on lipopolysaccharides(LPS)-stimulated murine RAW264.7 macrophages and explore its underlying mechanism of action.Methods:Total flavonoids from Saussurea involucrata were extracted using chromatographic column method.Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay.The production of nitric oxide was detected by Griess assay and the release of cytokines(IL-10 and TNF-α)and chemokines(MCP-1,MIP-1α,and CCL5/RANTES)was determined by ELISA to evaluate the anti-inflammatory activity of total flavonoids from Saussurea involucrata.Moreover,nuclear translocation of p65,c-Jun,and IRF3 was detected by immunofluorescence microscopy and Western blotting analysis was performed to determine the expression of related proteins.Results:Total flavonoids extracted from Saussurea involucrata were 751.5 mg/g and the content of rutin was 506.5 mg/g.The production of inflammatory mediators including nitric oxide,cytokines,and chemokines was effectively inhibited by total flavonoids from Saussurea involucrata.Meanwhile,total flavonoids also suppressed the nuclear translocation of p65,c-Jun,and IRF3 in LPS-stimulated RAW264.7 cells.The LPS-induced expression of iNOS and COX-2 was remarkably reduced by treatment with total flavonoids from Saussurea involucrata.Moreover,total flavonoids decreased the expression levels of p-IKKα/β,p-TBK1,p-p38,p-ERK,p-JNK,p-p65,p-c-Jun,and p-IRF3 in LPS-exposed RAW264.7 macrophages.Conclusions:Total flavonoids from Saussurea involucrata potentially inhibit the secretion of pro-inflammatory mediators,which may be related to inhibition of p65,c-Jun,and IRF3 signaling pathways in LPS-stimulated RAW264.7 cells. 关键词：Saussurea involucrata CYTOKINES PROINFLAMMATORY MACROPHAGES FLAVONOIDS

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Impact Factor:1.90 Total flavonoids from Saussurea involucrata attenuate inflammation in lipopolysaccharide-stimulated RAW264.7 macrophages via modulating p65,c-Jun,and IRF3 signaling pathways

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