Patterning the neuronal cells via inkjet printing of self-assembled peptides on silk scaffolds

查看全文 作　　者：Weizhen [1]Sun;Yi [1]Zhang;David [1,4]A.Gregory;Ana Jimenez-[1]Franco;Mhd Anas [1]Tomeh;Songwei [2]Lv;Jiqian [3]Wang;John [4]W.Haycock;Jian [5]R.Lu;Xiubo [1,2]Zhao

机构地区：[1]Department of Chemical and Biological Engineering,University of Sheffield,Sheffield,SI 3JD,UK;[2]School of Pharmacy,Changzhou University,Changzhou,213164,China;[3]Centre for Bioengineering and Biotechnology,China University of Petroleum(East China),Qingdao,266555,China;[4]Department of Materials Science&Engineering,University of Sheffield,Shejfield,SI 3JD,UK;[5]School of Physics and Astronomy,University of Manchester,Manchester,M139PL,UK

出　　处：《Progress in Natural Science:Materials International》索引2020年第30卷第5期,共11页

基　　金：the EPSRC (EP/N007174/1 and EP/N023579/1);Royal Society (RG160662 and IE150457);Jiangsu specially-appointed professor program for support。 摘　　要：The patterning of neuronal cells and guiding neurite growth are important for neuron tissue engineering and cell-based biosensors. In this paper, inkjet printing has been employed to pattern self-assembled I_3 QGK peptide nanofibers on silk substrates for guiding the growth of neuron-like PC12 cells. Atomic force microscopy(AFM)confirmed the dynamic self-assembly of I_3 QGK into nanofiber structures. The printed self-assembled peptide strongly adheres to regenerated silk fibroin(RSF) substrates through charge-charge interactions. It was observed that in the absence of I_3 QGK, PC12 cells exhibited poor attachment to RSF films, while for RSF surfaces coated or printed with peptide nanofibers, cellular attachment was significantly improved in terms of both cell density and morphology. AFM results revealed that peptide nanofibers can promote the generation of axons and terminal buttons of PC12 cells, indicating that I_3 QGK nanofibers not only promote cellular attachment but also facilitate differentiation into neuronal phenotypes. Inkjet printing allows complex patterning of peptide nanofibers onto RSF substrates, which enabled us to engineer cell alignment and provide an opportunity to direct axonal development in vitro. The live/dead assay showed that printed I_3 QGK patterns exhibit no cytotoxicity to PC12 cells demonstrating potential for future nerve tissue engineering applications. 关键词：NEUROBIOLOGY MICRO-PATTERNING Inkjet printing Self-assembling peptides PC12 cells

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Patterning the neuronal cells via inkjet printing of self-assembled peptides on silk scaffolds

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