African Swine Fever Virus MGF-110-9L-deficient Mutant Has Attenuated Virulence in Pigs

查看全文 作　　者：Dan [1]Li;Yinguang [1]Liu;Xiaolan [1]Qi;Yuan [1]Wen;Pan [1]Li;Zhao [1]Ma;Yongjie [1]Liu;Haixue [1]Zheng;Zhijie [1]Liu

机构地区：[1]State Key Laboratory of Veterinary Etiological Biology and OIE/National Foot and Mouth Disease Reference Laboratory,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China

出　　处：《Virologica Sinica》索引2021年第36卷第2期,共9页

基　　金：supported by grants from the National Key Research and Development Program(2018YFC0840402);National Natural Science Foundation of China(31941002);Special Fund for Basic Scientific Research of Chinese Academy of Agricultural Sciences (Y2019YJ07-01);Science and technology innovation engineering major scientific research program of Chinese Academy of Agricultural Sciences (CAASZDRW202006-03);State Key Laboratory of Veterinary Etiological Biology Major achievements cultivation project of Chinese Academy of Agricultural Sciences (SKLVEB2020CGPY02)。 摘　　要：African swine fever virus(ASFV)is the etiological agent of African swine fever(ASF),an often lethal disease in domestic and wild pigs.ASF represents a major threat to the swine industry worldwide.Currently,no commercial vaccine is available because of the complexity of ASFV or biosecurity concerns.Live attenuated viruses that are naturally isolated or genetically manipulated have demonstrated reliable protection against homologous ASFV strain challenge.In the present study,a mutant ASFV strain with the deletion of ASFV MGF-110-9L(ASFV-D9L)was generated from a highly virulent ASFV CN/GS/2018 parental strain,a genotypeⅡASFV.Relative to the parental ASFV isolate,deletion of the MGF-110-9L gene significantly decreased the ability of ASFV-D9L to replicate in vitro in primary swine macrophage cell cultures.The majority of animals inoculated intramuscularly with a low dose of ASFV-D9L(10 HAD50)remained clinically normal during the 21-day observational period.Three of five ASFV-D9L-infected animals displayed low viremia titers and low virus shedding and developed a strong virus-specific antibody response,indicating partial attenuation of the ASFV-D9L strain in pigs.The findings imply the potential usefulness of the ASFV-D9L strain for further development of ASF control measures. 关键词：African swine fever virus(ASFV) MGF-110-9L MUTANT Attenuated virulence PIG

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African Swine Fever Virus MGF-110-9L-deficient Mutant Has Attenuated Virulence in Pigs

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