The effect of LyPRP/collagen composite hydrogel on osteogenic differentiation of rBMSCs

查看全文 作　　者：Manyu [1]Chen;Quanying [1]Liu;Yang [1]Xu;Yuxiang [1]Wang;Xiaowen [1]Han;Zhe [2]Wang;Jie [1,3]Liang;Yong [1]Sun;Yujiang [1]Fan;Xingdong [1]Zhang

机构地区：[1]National Engineering Research Center for Biomaterials,Sichuan University,29 Wangjiang Road,Chengdu,Sichuan 610064,P.R.China;[2]Department of Medical Genetics,Zunyi Medical University,No.6 West Xuefu Road,Zunyi,Guizhou 563000,P.R.China;[3]Sichuan Testing Center for Biomaterials and Medical Devices,Sichuan Universit,29 Wangjiang Road,Chengdu 610064,P.R.China

出　　处：《Regenerative Biomaterials》索引2021年第8卷第1期,共11页

基　　金：This work was sponsored by National Key Research and Development Project of China(Grant No.2018YFC1106800);National Nature Science Foundation of China(Grant Nos 32071352 and 81860392);Sichuan Province Scientific and Technological Achievements Transformation and Guidance Project(Grant No.2016CZYD0004);Sichuan Province Key R&D Program(Grant No.2019YFS0007);Sichuan University Innovation Spark Project(Grant No.2018SCUH0089). 摘　　要：Although platelet-rich plasma(PRP)plays a significant role in the orthopedic clinical application,it still faces two major problems,namely,uncontrollable factors release,frequent preparation and extraction processes as well as the inconvenient form of usage.To overcome these shortcomings,freeze-dried PRP(LyPRP)was encapsulated into bioactive Col I hydrogel to induce osteogenic dif-ferentiation of rabbit bone marrow mesenchymal stem cells(rBMSCs).And PRP/Col I composite hydrogel was prepared as a control.Compared with Col I hydrogel,the introduction of platelets significantly improved the mechanical properties of hydrogels.Meanwhile,platelets were evenly distributed in the composite hydrogels network.The sustainable release of related factors in the composite hydrogels could last for more than 14days to maintain its long-term biological activity.Further cell experiments confirmed that PRP and LyPRP could effectively alleviate the contraction of collagen hydrogel in vitro,and promote the adhesion,proliferation and osteogenesis differentia-tion of rBMSCs.The results of osteogenic gene expression indicated that the 10%LyPRP/Col I composite hydrogel could facilitate the early expression of BMP-2 and late osteogenic associated protein formation with higher expression of alkaline phosphatase and Osteocalcin(OCN).These results might provide new insights for the clinical application of 10%LyPRP/Col I composite hydro-gel as practical bone repair injection. 关键词：platelet-rich plasma fee-dried platelet-rich plasma collagen hydrogel osteogenic dfferentiation

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The effect of LyPRP/collagen composite hydrogel on osteogenic differentiation of rBMSCs

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