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Effect of the number of freeze-thaw cycles number on the quality of Pacific white shrimp (Litopenaeus vannamei): An emphasis on moisture migration and microstructure by LF-NMR and SEM

查看全文 作  者:Weiqing [1,2,3,4]Lan;Xiaoyu [1]Hu;Xiaohong [1,2,3,4]Sun;Xi [1,2,3,4]Zhang;Jing [1,2,3,4]Xie 高影响力作者 机构地区:[1]College of Food Science and Technology,Shanghai Ocean University,Shanghai,201306,China;[2]Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center,Shanghai,201306,China;[3]Shanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation,Shanghai,201306,China;[4]National Experimental Teaching Demonstration Center for Food Science and Engineering(Shanghai Ocean University),Shanghai,201306,China高影响力机构 出  处:《Aquaculture and Fisheries》索引2020年第5卷第4期,共8页高影响力期刊 基  金:The study was financially supported by China Agricultural Research System(CARS-47-G26);Shanghai promote agriculture by applying scientific&technological advances projects(2016No.1-1);Ability promotion project of Shanghai Municipal Science and Technology Commission Engineering Center(16DZ2280300);Key Laboratory of Refrigeration and Conditioning Aquatic Products Processing,Ministry of Agriculture and Rural Affairs;The project was supported by Key Laboratory of Refrigeration and Conditioning Aquatic Products Processing,Ministry of Agriculture and Rural Affairs(Grant No.KLRCAPP2018-11). 摘  要:The objective of this study was to evaluate the effects of the number of freeze-thaw(F-T)cycles on moisture migration,protein degradation,microstructure and quality in Litopenaeus vannamei.The quality of samples with different F-T cycles were determined by low-field nuclear magnetic resonance(LF-NMR),magnetic resonance imaging(MRI)and scanning electron microscopy(SEM),combined with sodium dodecyl sulphate polyacrylamide gel electrophoresis(SDS-PAGE),texture properties analysis(TPA),color difference,polyphenol oxidase(PPO)activity,total volatile basic nitrogen(TVB-N),total viable count(TVC),and sensory evaluation.The results showed that F-T cycles caused a significantly increase in transverse relaxation time in T22 and T23 and a decreased brightness of pseudo-color diagram after 4 F-T cycles,indicating that water mobility increased as immobilized water was shifted to free water.The texture of samples declined as well,especially after 4 F-T cycles.The rapid growth of PPO activity caused a decrease in brightness(L*)and an increase in redness(a∗)after 3 F-T cycles.The increase of TVB-N,TVC,and sensory score explained the changes in shrimp quality which became obvious after 3 F-T cycles and unacceptable after 6 cycles.Meanwhile,fewer than 4 F-T cycles accelerated protein aggregation,while denaturation occurred after 4 cycles.Therefore,repeated F-T cycles could accelerate the changes of protein,microstructure,water distribution,and quality deterioration especially after 3 F-T cycles,with a threshold was 6 F-T cycles. 关 键 词:Litopenaeus vannamei Repetitive freeze-thaw Muscle microstructure Water migration Proteins
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