The construction of OPA1 overexpression plasmid and the cell model

查看全文 作　　者：Ma [1]Haojie;Gai [1]Cong;Feng [1]Wandi;Cheng [1]Cuicui;Zhou [1]Mengqi;Zhang [1]Jinkun;Zhang [1]Yuxin;Yang [1]Luping;Guo [1]Zhenyu;Sun [1]Hongmei 机构地区：[1]Department of Anatomy,School of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488 出　　处：《解剖学杂志》索引2021年第44卷第S01期,共1页 摘　　要：The purpose of this study is to construct OPA1 overexpression plasmid and transfected into human neuroblastoma SH-SY5Y cells to test the overexpression efficiency.Human neuroblastoma SH SY5Y cells were transfected with the OPA1 overexpression plasmid by Lipo3000 transfection reagent in liposome transduction technology.A total of 3 groups were set up:the control group,negative control group,and OPA1 overexpression model group.RT-PCR technique was used to detect the expression of OPA1 at mRNA level.In addition,Western blot technique was used to detect the expression of OPA1 at protein level.We found that the mRNA and protein expression of OPA1 were significantly increased after transfection with OPA1 overexpression plasmid.These results highlight using liposome transduction technology,the OPA1 overexpression plasmid was successfully transfected into SH-SY5Y cells by Lipo3000 and the cell model of OPA1 gene overexpression was successully established. 关 键 词：OPA1 NEUROBLASTOMA technology.