维普中文期刊产品整合服务

Preparation of hapten-specific monoclonal antibody for cadmium and its ELISA application to aqueous samples

查看全文 作　　者：Huan [1]HE;Bo [2]TANG;Cheng [1]SUN;Shaogui [1]YANG;Weijuan [2]ZHENG;Zichun [2]HUA 高影响力作者 机构地区：[1]The State Key Laboratory of Pollution Control and Resource Reuse,School of the Environment,Nanjing University,Nanjing 210093,China;[2]The State Key Laboratory of Pharmaceutical Biotechnology,School of Life Science,Nanjing University,Nanjing 210093,China高影响力机构 出　　处：《Frontiers of Environmental Science & Engineering》索引2011年第5卷第3期,共8页高影响力期刊 基　　金：This work was supported by the National High Technology Research and Development Program of China(No.2006AA06Z411);China Postdoctoral Science Foundation(No.20100471297);Jiangsu Planned Projects for Postdoctoral Research Funds(No.0902065C). 摘　　要：High-affinity and specific monoclonal antibodies against cadmium-ethylene diamine tetraacetic acid(EDTA)complex have been produced using the hybridoma technique.A hapten was synthesized and characterized by Fourier Transform Infrared Spectroscopy(FT-IR)and UVVis.Competitive enzyme-linked immunosorbent assay(ELISA)for quantitative detection of cadmium in aqueous sample was developed.The monoclonal antibody with high level of binding affinity for Cd-IEDTA-BSA and high specificity for soluble Cd-EDTA complex showed less than 0.99%cross-reactivity with other 11 metals.The limit of detection was 0.10μg·L^(-1),and the effective linear range was 10^(-1)-10^(3)μg·L^(-1).The intra-and inter-assay coefficient variations were 1.5%-6.3%and 3.2%-7.4%,respectively.The spike recovery in different water samples were between 98.5%and 110.3%.The detection limit of this assay was well below the allowable concentration of cadmium(3μg·L^(-1)),and the working range was wider than that of other methods which showed the range of 2.19-86.38 and 0-10^(3)μg·L^(-1).The competitive ELISA established in this paper was sensitive and accurate in the screening of cadmium in aqueous samples.The results will lay a solid foundation for construction of an immunoassay kit for cadmium. 关 键 词：CADMIUM HAPTEN monoclonal antibody enzyme-linked immunosorbent assay(ELISA)
相关文献

参考文献(36)

引证文献(4)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费