Target Extension-Activated DNA Walker on Nanoparticles for Digital Counting-Based Analysis of MicroRNA

查看全文 作　　者：Qinya [1]Feng;Yuqing [1]Zhai;Wei [1]Ren;Chenghui [1]Liu

机构地区：[1]Key Laboratory of Applied Surface and Colloid Chemistry,Ministry of Education,Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province,School of Chemistry&Chemical Engineering,Shaanxi Normal University,Xi'an,Shaanxi 710119,China

出　　处：《Chinese Journal of Chemistry》索引2021年第39卷第6期,共6页

基　　金：the National Natural Science Foundation of China(Nos.22074088 and 21904083);the Program for Changjiang Scholars and Innovative Research Team in University(IRT_15R43);the Fundamental Research Funds for the Central Universities(Nos.GK202003038,GK201901003,2020TS089,GK202101001). 摘　　要：MicroRNAs(miRNAs),especially exosomal miRNAs,are promising noninvasive biomarkers in early-stage cancer diagnosis and disease treatment monitoring.However,their precise and sensitive quantification remains challenging due to their small size and low abundance.Herein,we have developed a nanoparticle-confined DNA walker strategy for the specific detection of miRNA.In the existence of the target miRNA;the on-particle DNA walking reaction will be initiated,providing a fluorescence-positive nanoparticle.Otherwise,the nanoparticle would be fluorescence-negative.Utilizing the total internal reflection fluorescent microscope(TIRFM)to digitally count the fluorescence-positive nanoparticles,the proposed method possesses a detection limit of 0.2 pmol/L miRNA and can accurately distinguish the single-base mismatched target.This design combines the merits of the DNA walker for signal amplification and the TIRFM for highly sensitive detection,paving a new way for the digital counting-based analysis of exosomal miRNAs. 关键词：RNA recognition DNA cleavage Digital counting Total internal reflection fluorescent microscope NANOPARTICLES

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Target Extension-Activated DNA Walker on Nanoparticles for Digital Counting-Based Analysis of MicroRNA

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